Possible involvement of folliculo-stellate cells in the differentiation of muscle fibers during monolayer culture of pituitary cells

Osamu Hosoya, Y. G. Watanabe

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A major objective of the present study was to examine the possibility that non-granular folliculo-stellate (FS) cells in the rat anterior pituitary are involved in the myogenesis that occurs during pituitary cell culture. Enzymatically dissociated anterior pituitary cells were fractionated by use of the Percoll gradient method. The proportion of FS cells was 5.8% on average before cell fractionation. After employing the Percoll gradient procedure, FS cells were enriched to a ratio of 12.2%. Three of five cell fractions were separately cultured, and the incidence of striated muscle fibers was quantitatively investigated. There was a good correlation between the numbers of muscle fibers and the proportions of FS cells in the fractions obtained from the Percoll gradient. These results suggest that FS cells are the cells that transform into striated muscles in pituitary monolayer cultures.

Original languageEnglish
Pages (from-to)141-145
Number of pages5
JournalZoological Science
Volume14
Issue number1
Publication statusPublished - 1997
Externally publishedYes

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muscle fibers
cell differentiation
cells
striated muscle
anterior pituitary
cell fractionation
muscle development
cell culture
incidence
rats

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Possible involvement of folliculo-stellate cells in the differentiation of muscle fibers during monolayer culture of pituitary cells. / Hosoya, Osamu; Watanabe, Y. G.

In: Zoological Science, Vol. 14, No. 1, 1997, p. 141-145.

Research output: Contribution to journalArticle

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