Possible Involvement of Convergent Nociceptive Input to Medullary Dorsal Horn Neurons in Intraoral Hyperalgesia Following Peripheral Nerve Injury

Ryuji Terayama, Hiroki Tsuchiya, Shinji Omura, Kotaro Maruhama, Masahide Mizutani, Seiji Iida, Tomosada Sugimoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Previous studies demonstrated that the number of c-Fos protein-like immunoreactive (c-Fos-IR) neurons in the medullary dorsal horn (MDH) evoked by noxious stimulation was increased after peripheral nerve injury, and such increase has been proposed to reflect the development of neuropathic pain state. The aim of this study was to examine the MDH for convergent collateral primary afferent input to second order neurons deafferented by peripheral nerve injury, and to explore a possibility of its contribution to the c-Fos hyperinducibility. Double immunofluorescence labeling for c-Fos and phosphorylated extracellular signal-regulated kinase (p-ERK) was performed to detect convergent synaptic input. c-Fos expression and the phosphorylation of ERK were induced by the intraoral application of capsaicin and by electrical stimulation of the inferior alveolar nerve (IAN), respectively. The number of c-Fos-IR neurons in the MDH induced by the intraoral application of capsaicin was increased after IAN injury, whereas the number of p-ERK immunoreactive neurons remained unchanged. The number of double-labeled neurons, that presumably received convergent primary afferent input from the lingual nerve and the IAN, was significantly increased after IAN injury. These results indicated that convergent primary nociceptive input through neighboring intact nerves may contribute to the c-Fos hyperinducibility in the MDH and the pathogenesis of neuropathic pain following trigeminal nerve injury.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalCellular and Molecular Neurobiology
Volume35
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Posterior Horn Cells
Peripheral Nerve Injuries
Hyperalgesia
Mandibular Nerve
Neurons
Proto-Oncogene Proteins c-fos
Capsaicin
Extracellular Signal-Regulated MAP Kinases
Neuralgia
Trigeminal Nerve Injuries
Lingual Nerve
Wounds and Injuries
Electric Stimulation
Fluorescent Antibody Technique
Phosphorylation
Spinal Cord Dorsal Horn

Keywords

  • C-Fos
  • ERK
  • Immunohistochemistry
  • Medullary dorsal horn
  • Orofacial pain
  • Trigeminal nerve

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Medicine(all)

Cite this

Possible Involvement of Convergent Nociceptive Input to Medullary Dorsal Horn Neurons in Intraoral Hyperalgesia Following Peripheral Nerve Injury. / Terayama, Ryuji; Tsuchiya, Hiroki; Omura, Shinji; Maruhama, Kotaro; Mizutani, Masahide; Iida, Seiji; Sugimoto, Tomosada.

In: Cellular and Molecular Neurobiology, Vol. 35, No. 3, 2015, p. 417-423.

Research output: Contribution to journalArticle

Terayama, Ryuji ; Tsuchiya, Hiroki ; Omura, Shinji ; Maruhama, Kotaro ; Mizutani, Masahide ; Iida, Seiji ; Sugimoto, Tomosada. / Possible Involvement of Convergent Nociceptive Input to Medullary Dorsal Horn Neurons in Intraoral Hyperalgesia Following Peripheral Nerve Injury. In: Cellular and Molecular Neurobiology. 2015 ; Vol. 35, No. 3. pp. 417-423.
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