Possible evidence of contamination by catechins in deconjugation enzymes from Helix pomatia and Abalone entrails

Toshiyuki Nakamura, Ryohei Tanaka, Hitoshi Ashida

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

β-Glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. In the present study, we found that compounds having the same molecular weights as catechins were present in Helix pomatia- and/or Abalone entrails-derived μ-glucuronidase and sulfatase by liquid chromatography tandem mass spectrometry (LC-MS/MS) with multiple reaction monitoring methods. On the other hand, the same molecular weights as catechins were undetectable in Escherichia coli-derived μ-glucuronidase and Aerobacter aerogenes-derived sulfatase. By high performance liquid chromatography, enzyme-derived catechins were not detected because of approximately 1,000-fold lower sensitivity as compared to LC-MS/MS. These results suggest that the catechins in these enzymes might be attributed to the diets of the organisms as the enzyme sources.

Original languageEnglish
Pages (from-to)1506-1510
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number8
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Catechin
Sulfatases
Glucuronidase
Contamination
Enzymes
Molecular Weight
Molecular weight
Enterobacter aerogenes
Glucuronic Acid
Liquid chromatography
High performance liquid chromatography
Polyphenols
Nutrition
Tandem Mass Spectrometry
Liquid Chromatography
Escherichia coli
Sulfates
Mass spectrometry
High Pressure Liquid Chromatography
Diet

Keywords

  • β-glucuronidase
  • Catechin
  • Liquid chromatography tandem mass spectrometry (LC-MS/MS)
  • Sulfatase

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Possible evidence of contamination by catechins in deconjugation enzymes from Helix pomatia and Abalone entrails. / Nakamura, Toshiyuki; Tanaka, Ryohei; Ashida, Hitoshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 8, 2011, p. 1506-1510.

Research output: Contribution to journalArticle

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