Possible coupling of prostaglandin E receptor EP1 to TRP5 expressed in Xenopus laevis oocytes

Hiroyuki Tabata, Satoshi Tanaka, Yukihiko Sugimoto, Hideaki Kanki, Shuji Kaneko, Atsushi Ichikawa

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We previously reported that the prostaglandin E2 (PGE2) receptor subtype EP1 is coupled to intracellular Ca2+ mobilization in CHO cells, which is dependent on extracellular Ca2+ in a pertussis toxin-insensitive manner [H. Katoh, et al., Biochim. Biophys. Acta 1244 (1995) 41-48]. However, it remains unknown about the signal transduction involved in this response. To investigate the mechanism regulating Ca2+ mobilization mediated by EP1 receptors in detail, we performed a series of experiments using the Xenopus laevis oocyte expression system and found that endogenous Gq and/or G11, and not Gi1 is involved in the Ca2+ mobilization induced by PGE2. We further investigated the receptor-activated Ca2+ channel (RACC)-related response by introducing mRNA for mouse transient receptor potential 5 (TRP5), a possible candidate for the RACC, and found effective coupling between them. These results suggest that the EP1 receptors induce Ca2+ mobilization via Gq and/or G11 and Ca2+ influx via TRP.

Original languageEnglish
Pages (from-to)398-402
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume298
Issue number3
DOIs
Publication statusPublished - Nov 25 2002

Keywords

  • Ca mobilization
  • EP
  • Receptor-activated Ca channel
  • Transient receptor potential

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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