Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation

Hiroaki Inaba, Hideyuki Sugita, Masae Kuboniwa, Soichi Iwai, Masakazu Hamada, Takeshi Noda, Ichijiro Morisaki, Richard J. Lamont, Atsuo Amano

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Summary: Recent epidemiological studies have revealed a significant association between periodontitis and oral squamous cell carcinoma (OSCC). Furthermore, matrix metalloproteinase 9 (MMP9) is implicated in the invasion and metastasis of tumour cells. We examined the involvement of Porphyromonas gingivalis, a periodontal pathogen, in OSCC invasion through induced expression of proMMP and its activation. proMMP9 was continuously secreted from carcinoma SAS cells, while P.gingivalis infection increased proenzyme expression and subsequently processed it to active MMP9 in culture supernatant, which enhanced cellular invasion. In contrast, Fusobacterium nucleatum, another periodontal organism, failed to demonstrate such activities. The effects of P.gingivalis were observed with highly invasive cells, but not with the low invasivetype. P.gingivalis also stimulated proteinase-activated receptor 2 (PAR2) and enhanced proMMP9 expression, which promoted cellular invasion. P.gingivalis mutants deficient in gingipain proteases failed to activate MMP9. Infected SAS cells exhibited activation of ERK1/2, p38, and NF-kB, and their inhibitors diminished both proMMP9-overexpression and cellular invasion. Together, our results show that P.gingivalis activates the ERK1/2-Ets1, p38/HSP27, and PAR2/NF-kB pathways to induce proMMP9 expression, after which the proenzyme is activated by gingipains to promote cellular invasion of OSCC cell lines. These findings suggest a novel mechanism of progression and metastasis of OSCC associated with periodontitis.

Original languageEnglish
Pages (from-to)131-145
Number of pages15
JournalCellular Microbiology
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 2014
Externally publishedYes

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Porphyromonas gingivalis
Squamous Cell Carcinoma
Matrix Metalloproteinase 9
PAR-2 Receptor
Enzyme Precursors
NF-kappa B
Periodontitis
Fusobacterium nucleatum
Neoplasm Metastasis
Epidemiologic Studies
Peptide Hydrolases
Carcinoma
Cell Line
Infection
Neoplasms
Porphyromonas gingivalis argingipain

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation. / Inaba, Hiroaki; Sugita, Hideyuki; Kuboniwa, Masae; Iwai, Soichi; Hamada, Masakazu; Noda, Takeshi; Morisaki, Ichijiro; Lamont, Richard J.; Amano, Atsuo.

In: Cellular Microbiology, Vol. 16, No. 1, 01.2014, p. 131-145.

Research output: Contribution to journalArticle

Inaba, H, Sugita, H, Kuboniwa, M, Iwai, S, Hamada, M, Noda, T, Morisaki, I, Lamont, RJ & Amano, A 2014, 'Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation', Cellular Microbiology, vol. 16, no. 1, pp. 131-145. https://doi.org/10.1111/cmi.12211
Inaba, Hiroaki ; Sugita, Hideyuki ; Kuboniwa, Masae ; Iwai, Soichi ; Hamada, Masakazu ; Noda, Takeshi ; Morisaki, Ichijiro ; Lamont, Richard J. ; Amano, Atsuo. / Porphyromonas gingivalis promotes invasion of oral squamous cell carcinoma through induction of proMMP9 and its activation. In: Cellular Microbiology. 2014 ; Vol. 16, No. 1. pp. 131-145.
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