Porous zirconia/hydroxyapatite scaffolds for bone reconstruction

Sang Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Atsushi Nakahira, Kyo Han Kim, Satoshi Imazato

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Objective: Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect. Methods: To address the hypothesis that using a ZrO 2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO 2/HAp composite scaffolds with different ZrO 2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO 2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material. Results: The porosity of a ZrO 2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO 2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO 2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO 2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO 2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material. Significance: We thus showed that a porous ZrO 2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects.

Original languageEnglish
Pages (from-to)1221-1231
Number of pages11
JournalDental Materials
Volume28
Issue number12
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Durapatite
Hydroxyapatite
Scaffolds
Zirconia
Bone
Bone and Bones
Bone Regeneration
Mesenchymal Stromal Cells
Cell Adhesion
Cell adhesion
Defects
Cell Proliferation
Compressive Strength
Composite materials
Apatites
Histocompatibility
zirconium oxide
Porosity
Tissue
Bioceramics

Keywords

  • Bone marrow derived stromal cells
  • Bone tissue engineering
  • Hydroxyapatite
  • Porous scaffold
  • Zirconia

ASJC Scopus subject areas

  • Dentistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Medicine(all)

Cite this

An, S. H., Matsumoto, T., Miyajima, H., Nakahira, A., Kim, K. H., & Imazato, S. (2012). Porous zirconia/hydroxyapatite scaffolds for bone reconstruction. Dental Materials, 28(12), 1221-1231. https://doi.org/10.1016/j.dental.2012.09.001

Porous zirconia/hydroxyapatite scaffolds for bone reconstruction. / An, Sang Hyun; Matsumoto, Takuya; Miyajima, Hiroyuki; Nakahira, Atsushi; Kim, Kyo Han; Imazato, Satoshi.

In: Dental Materials, Vol. 28, No. 12, 12.2012, p. 1221-1231.

Research output: Contribution to journalArticle

An, SH, Matsumoto, T, Miyajima, H, Nakahira, A, Kim, KH & Imazato, S 2012, 'Porous zirconia/hydroxyapatite scaffolds for bone reconstruction', Dental Materials, vol. 28, no. 12, pp. 1221-1231. https://doi.org/10.1016/j.dental.2012.09.001
An, Sang Hyun ; Matsumoto, Takuya ; Miyajima, Hiroyuki ; Nakahira, Atsushi ; Kim, Kyo Han ; Imazato, Satoshi. / Porous zirconia/hydroxyapatite scaffolds for bone reconstruction. In: Dental Materials. 2012 ; Vol. 28, No. 12. pp. 1221-1231.
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