Porous zirconia/hydroxyapatite scaffolds for bone reconstruction

Sang Hyun An, Takuya Matsumoto, Hiroyuki Miyajima, Atsushi Nakahira, Kyo Han Kim, Satoshi Imazato

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Objective: Highly porous apatite-based bioceramic scaffolds have been widely investigated as three-dimensional (3D) templates for cell adhesion, proliferation, and differentiation promoting the bone regeneration. Their fragility, however, limits their clinical application especially for a large bone defect. Methods: To address the hypothesis that using a ZrO 2/hydroxyapatite (HAp) composite might improve both the mechanical properties and cellular compatibility of the porous material, we fabricated ZrO 2/HAp composite scaffolds with different ZrO 2/HAp ratios, and evaluated their characteristics. In addition, porous ZrO 2/HAp scaffolds containing bone marrow derived stromal cells (BMSCs) were implanted into critical-size bone defects for 6 weeks in order to evaluate the bone tissue reconstruction with this material. Results: The porosity of a ZrO 2/HAp scaffold can be adjusted from 72% to 91%, and the compressive strength of the scaffold increased from 2.5 to 13.8 MPa when the ZrO 2 content increased from 50 to 100 wt%. The cell adhesion and proliferation in the ZrO 2/HAp scaffold was greatly improved when compared to the scaffold made with ZrO 2 alone. Moreover, in vivo study showed that a BMSCs-loaded ZrO 2/HAp scaffold provided a suitable 3D environment for BMSC survival and enhanced bone regeneration around the implanted material. Significance: We thus showed that a porous ZrO 2/HAp composite scaffold has excellent mechanical properties, and cellular/tissue compatibility, and would be a promising substrate to achieve both bone reconstruction and regeneration needed in the treatment of large bone defects.

Original languageEnglish
Pages (from-to)1221-1231
Number of pages11
JournalDental Materials
Volume28
Issue number12
DOIs
Publication statusPublished - Dec 1 2012

Keywords

  • Bone marrow derived stromal cells
  • Bone tissue engineering
  • Hydroxyapatite
  • Porous scaffold
  • Zirconia

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

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  • Cite this

    An, S. H., Matsumoto, T., Miyajima, H., Nakahira, A., Kim, K. H., & Imazato, S. (2012). Porous zirconia/hydroxyapatite scaffolds for bone reconstruction. Dental Materials, 28(12), 1221-1231. https://doi.org/10.1016/j.dental.2012.09.001