TY - GEN
T1 - Porous scaffolds consisting of collagen, chondroitin sulfate, and hydroxyapatite with enhanced biodegradable resistance for cartilage regeneration
AU - Kaneda, H.
AU - Ikoma, T.
AU - Yoshioka, T.
AU - Nishi, M.
AU - Matsumoto, R.
AU - Uemura, T.
AU - Cross, J. S.
AU - Tanaka, J.
PY - 2011
Y1 - 2011
N2 - Porous scaffolds of alkaline-soluble collagen including nanocomposite particles of chondroitin sulfate and low crystalline hydroxyapatite for cartilage regeneration were fabricated by freeze-drying and thermal dehydration treatments; porous collagen scaffolds were also synthesized as a reference. The scaffolds were cross-linked using glutaraldehyde (GA) vapor treatment in order to enhance biodegradable resistance. Microstructural observation with scanning electron microscope indicated that the scaffolds with and without GA cross-linkage had open pores between 130 to 200 μm in diameter and well-interconnected pores of 10 to 30 μm even after cross-linkage. In vitro biodegradable resistance to collagenase was significantly enhanced by GA cross-linking of the scaffolds. All these results suggest that the GA cross-linked scaffolds consisting of collagen, chondroitin sulfate, and low crystalline hydroxyapatite have suitable microporous structures and long-term biochemical stability for cartilage tissue engineering.
AB - Porous scaffolds of alkaline-soluble collagen including nanocomposite particles of chondroitin sulfate and low crystalline hydroxyapatite for cartilage regeneration were fabricated by freeze-drying and thermal dehydration treatments; porous collagen scaffolds were also synthesized as a reference. The scaffolds were cross-linked using glutaraldehyde (GA) vapor treatment in order to enhance biodegradable resistance. Microstructural observation with scanning electron microscope indicated that the scaffolds with and without GA cross-linkage had open pores between 130 to 200 μm in diameter and well-interconnected pores of 10 to 30 μm even after cross-linkage. In vitro biodegradable resistance to collagenase was significantly enhanced by GA cross-linking of the scaffolds. All these results suggest that the GA cross-linked scaffolds consisting of collagen, chondroitin sulfate, and low crystalline hydroxyapatite have suitable microporous structures and long-term biochemical stability for cartilage tissue engineering.
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U2 - 10.1557/opl.2011.535
DO - 10.1557/opl.2011.535
M3 - Conference contribution
AN - SCOPUS:84861126616
SN - 9781605112787
T3 - Materials Research Society Symposium Proceedings
SP - 117
EP - 123
BT - Soft Matter, Biological Materials and Biomedical Materials - Synthesis, Characterization and Applications
T2 - 2010 MRS Fall Meeting
Y2 - 29 November 2010 through 3 December 2010
ER -