Porous scaffolds consisting of collagen, chondroitin sulfate, and hydroxyapatite with enhanced biodegradable resistance for cartilage regeneration

H. Kaneda, T. Ikoma, Tomohiko Yoshioka, M. Nishi, R. Matsumoto, T. Uemura, J. S. Cross, J. Tanaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Porous scaffolds of alkaline-soluble collagen including nanocomposite particles of chondroitin sulfate and low crystalline hydroxyapatite for cartilage regeneration were fabricated by freeze-drying and thermal dehydration treatments; porous collagen scaffolds were also synthesized as a reference. The scaffolds were cross-linked using glutaraldehyde (GA) vapor treatment in order to enhance biodegradable resistance. Microstructural observation with scanning electron microscope indicated that the scaffolds with and without GA cross-linkage had open pores between 130 to 200 μm in diameter and well-interconnected pores of 10 to 30 μm even after cross-linkage. In vitro biodegradable resistance to collagenase was significantly enhanced by GA cross-linking of the scaffolds. All these results suggest that the GA cross-linked scaffolds consisting of collagen, chondroitin sulfate, and low crystalline hydroxyapatite have suitable microporous structures and long-term biochemical stability for cartilage tissue engineering.

Original languageEnglish
Title of host publicationMaterials Research Society Symposium Proceedings
Pages117-123
Number of pages7
Volume1301
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event2010 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 29 2010Dec 3 2010

Other

Other2010 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/29/1012/3/10

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ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

Kaneda, H., Ikoma, T., Yoshioka, T., Nishi, M., Matsumoto, R., Uemura, T., Cross, J. S., & Tanaka, J. (2011). Porous scaffolds consisting of collagen, chondroitin sulfate, and hydroxyapatite with enhanced biodegradable resistance for cartilage regeneration. In Materials Research Society Symposium Proceedings (Vol. 1301, pp. 117-123) https://doi.org/10.1557/opl.2011.535