TY - JOUR
T1 - Population analysis of cellular responses to synthetic peptides of Der p II, a major allerg molecule of Dermatophagoides pteronyssinus, in allergic and nonallergic subjects
AU - Okano, M.
AU - Nagano, T.
AU - Kino, K.
AU - Yasueda, H.
AU - Baba, Y.
AU - Saito, C.
AU - Masuda, Y.
AU - Ohta, N.
PY - 1994/7
Y1 - 1994/7
N2 - Okano M, Nagano T, Kino K, Yasueda H, Baba Y, Saito C, Masuda Y, Ohta N. Population analysis of cellular responses to synthetic peptides of Der p II, a major allergen molecule of Dermatophagoides pteronyssinus, in allergic and nonallergic subjects. Responses of peripheral blood mononuclear cells to synthetic oligopeptides of Der p II, one of the major allergen molecules of Dermatophagoides pteronyssinus, were compared between allergic and nonallergic subjects. Healthy subjects showed positive responses to crude extracts of D. pteronyssinus, but only allergic subjects showed elevated cellular responses to Der p II. We synthesized three oligopeptides of Der p II in which motifs of a possible T‐cell epitopc were included. Of 14 subjects with positive response to Der p II, three responded to all three peptides, while five did not respond to any peptide tested. In 11 allergic patients who showed positive response to Der p II, responsiveness to the peptide K33‐T47 was significantly higher than that to other peptides (P<0.05). All the responding patients were also positive for scratch test to Der p II, suggesting that those epitopcs induced IgE‐promoting helper T‐cell response in allergic persons. On the other hand, the in vitro cellular responses were not necessarily correlated to IgE production against Der p II in healthy subjects.
AB - Okano M, Nagano T, Kino K, Yasueda H, Baba Y, Saito C, Masuda Y, Ohta N. Population analysis of cellular responses to synthetic peptides of Der p II, a major allergen molecule of Dermatophagoides pteronyssinus, in allergic and nonallergic subjects. Responses of peripheral blood mononuclear cells to synthetic oligopeptides of Der p II, one of the major allergen molecules of Dermatophagoides pteronyssinus, were compared between allergic and nonallergic subjects. Healthy subjects showed positive responses to crude extracts of D. pteronyssinus, but only allergic subjects showed elevated cellular responses to Der p II. We synthesized three oligopeptides of Der p II in which motifs of a possible T‐cell epitopc were included. Of 14 subjects with positive response to Der p II, three responded to all three peptides, while five did not respond to any peptide tested. In 11 allergic patients who showed positive response to Der p II, responsiveness to the peptide K33‐T47 was significantly higher than that to other peptides (P<0.05). All the responding patients were also positive for scratch test to Der p II, suggesting that those epitopcs induced IgE‐promoting helper T‐cell response in allergic persons. On the other hand, the in vitro cellular responses were not necessarily correlated to IgE production against Der p II in healthy subjects.
KW - Der p II
KW - Dermatophagoidesspp.
KW - T epitope
KW - house‐dust mite allergy, human T cells, synthetic peptides
UR - http://www.scopus.com/inward/record.url?scp=0028243998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028243998&partnerID=8YFLogxK
U2 - 10.1111/j.1398-9995.1994.tb00836.x
DO - 10.1111/j.1398-9995.1994.tb00836.x
M3 - Article
C2 - 7521142
AN - SCOPUS:0028243998
VL - 49
SP - 436
EP - 441
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 6
ER -