Population analysis of cellular responses to synthetic peptides of Der p II, a major allerg molecule of Dermatophagoides pteronyssinus, in allergic and nonallergic subjects

M. Okano, T. Nagano, K. Kino, H. Yasueda, Y. Baba, C. Saito, Y. Masuda, N. Ohta

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Okano M, Nagano T, Kino K, Yasueda H, Baba Y, Saito C, Masuda Y, Ohta N. Population analysis of cellular responses to synthetic peptides of Der p II, a major allergen molecule of Dermatophagoides pteronyssinus, in allergic and nonallergic subjects. Responses of peripheral blood mononuclear cells to synthetic oligopeptides of Der p II, one of the major allergen molecules of Dermatophagoides pteronyssinus, were compared between allergic and nonallergic subjects. Healthy subjects showed positive responses to crude extracts of D. pteronyssinus, but only allergic subjects showed elevated cellular responses to Der p II. We synthesized three oligopeptides of Der p II in which motifs of a possible T‐cell epitopc were included. Of 14 subjects with positive response to Der p II, three responded to all three peptides, while five did not respond to any peptide tested. In 11 allergic patients who showed positive response to Der p II, responsiveness to the peptide K33‐T47 was significantly higher than that to other peptides (P<0.05). All the responding patients were also positive for scratch test to Der p II, suggesting that those epitopcs induced IgE‐promoting helper T‐cell response in allergic persons. On the other hand, the in vitro cellular responses were not necessarily correlated to IgE production against Der p II in healthy subjects.

Original languageEnglish
Pages (from-to)436-441
Number of pages6
JournalAllergy
Volume49
Issue number6
DOIs
Publication statusPublished - Jul 1994

Keywords

  • Der p II
  • Dermatophagoidesspp.
  • T epitope
  • house‐dust mite allergy, human T cells, synthetic peptides

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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