Polymorphisms of E1 and GIGANTEA in wild populations of Lotus japonicus

Tomomi Wakabayashi, Hana Oh, Masayoshi Kawaguchi, Kyuya Harada, Shusei Sato, Hajime Ikeda, Setoguchi Hiroaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In plants, timing of flowering is an essential factor that controls the survival rates of descendants. The circadian clock genes E1 and GIGANTEA (GI) play a central role in transmitting signals to FLOWERING LOCUS T (FT) in leguminous plants. Lotus japonicus is a wild Japanese species that ranges from northern Hokkaido to the southern Ryukyus and exhibits a wide range in terms of the time between seeding and first flowering. In this study, we first identified LjGI and analyzed polymorphisms of LjE1 and LjGI among wild populations covering the entire distribution range of this species in Japan. LjGI had a coding sequence (CDS) length of 3495 bp and included 14 exons. The homologies of DNA and amino acid sequences between LjGI and GmGI were 89 and 88 % (positive rate was 92 %), respectively. LjE1 harbored five nucleic acid changes in a 552 bp CDS, all of which were nonsynonymous; four of the changes were located in the core function area. LjE1 alleles exhibited partial north–south differentiation and non-neutrality. In contrast, the LjGI harbored one synonymous and one nonsynonymous change. Thus, our study suggests that LjE1 may be involved in the control of flowering times, whereas LjGI may be under strong purifying selection.

Original languageEnglish
Pages (from-to)651-660
Number of pages10
JournalJournal of Plant Research
Volume127
Issue number6
DOIs
Publication statusPublished - Oct 30 2014

Fingerprint

Lotus corniculatus var. japonicus
genetic polymorphism
flowering
Amino Acid Sequence Homology
Circadian Clocks
Japan
Nucleic Acids
Population
Exons
Alleles
circadian rhythm
nucleic acids
exons
DNA
amino acid sequences
sowing
survival rate
Genes
alleles
Loteae

Keywords

  • Circadian clock
  • E1
  • GIGANTEA
  • Lotus japonicus
  • Nonsynonymous change

ASJC Scopus subject areas

  • Plant Science
  • Medicine(all)

Cite this

Wakabayashi, T., Oh, H., Kawaguchi, M., Harada, K., Sato, S., Ikeda, H., & Hiroaki, S. (2014). Polymorphisms of E1 and GIGANTEA in wild populations of Lotus japonicus. Journal of Plant Research, 127(6), 651-660. https://doi.org/10.1007/s10265-014-0649-8

Polymorphisms of E1 and GIGANTEA in wild populations of Lotus japonicus. / Wakabayashi, Tomomi; Oh, Hana; Kawaguchi, Masayoshi; Harada, Kyuya; Sato, Shusei; Ikeda, Hajime; Hiroaki, Setoguchi.

In: Journal of Plant Research, Vol. 127, No. 6, 30.10.2014, p. 651-660.

Research output: Contribution to journalArticle

Wakabayashi, T, Oh, H, Kawaguchi, M, Harada, K, Sato, S, Ikeda, H & Hiroaki, S 2014, 'Polymorphisms of E1 and GIGANTEA in wild populations of Lotus japonicus', Journal of Plant Research, vol. 127, no. 6, pp. 651-660. https://doi.org/10.1007/s10265-014-0649-8
Wakabayashi, Tomomi ; Oh, Hana ; Kawaguchi, Masayoshi ; Harada, Kyuya ; Sato, Shusei ; Ikeda, Hajime ; Hiroaki, Setoguchi. / Polymorphisms of E1 and GIGANTEA in wild populations of Lotus japonicus. In: Journal of Plant Research. 2014 ; Vol. 127, No. 6. pp. 651-660.
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