Polymorphism of the D12S391 microsatellite in a Japanese population sample

Y. Shigeta; Y. Yamamoto; Y. Doi; S. Miyaishi; H. Ishizu

doi:10.1016/S0379-0738(99)00048-1

Polymorphism of the D12S391 microsatellite in a Japanese population sample

Y. Shigeta, Y. Yamamoto, Y. Doi, S. Miyaishi, H. Ishizu

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Using the polymerase chain reaction (PCR), we studied the short tandem repeat (STR) polymorphism observed at the D12S391 locus. In 350 Japanese examined, 14 different alleles ranging from 209 bp to 261 bp were detected. Allele 18 (221 bp) showed the highest frequency at 0.30. Observed and expected values of respective genotypes satisfied the Hardy-Weinberg equilibrium (χ² = 24.08, P = 0.24, df = 20). In addition, 18 additional sequence structures (suballeles), were detected in this study. Within the suballeles, sequence variants, in which the initial repeat of (AGAT) was replaced with (AGGT), was found in five samples. It was found that the analysis of single-strand conformation polymorphism (SSCP) before sequence analysis was useful for distinguishing these suballeles.

Original language	English
Pages (from-to)	61-66
Number of pages	6
Journal	Forensic Science International
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/S0379-0738(99)00048-1
Publication status	Published - May 31 1999

Keywords

D12S391
Microsatellite polymorphism
SSCP analysis
STR
Sequence analysis
Suballeles

ASJC Scopus subject areas

Pathology and Forensic Medicine

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title = "Polymorphism of the D12S391 microsatellite in a Japanese population sample",

abstract = "Using the polymerase chain reaction (PCR), we studied the short tandem repeat (STR) polymorphism observed at the D12S391 locus. In 350 Japanese examined, 14 different alleles ranging from 209 bp to 261 bp were detected. Allele 18 (221 bp) showed the highest frequency at 0.30. Observed and expected values of respective genotypes satisfied the Hardy-Weinberg equilibrium (χ2 = 24.08, P = 0.24, df = 20). In addition, 18 additional sequence structures (suballeles), were detected in this study. Within the suballeles, sequence variants, in which the initial repeat of (AGAT) was replaced with (AGGT), was found in five samples. It was found that the analysis of single-strand conformation polymorphism (SSCP) before sequence analysis was useful for distinguishing these suballeles.",

keywords = "D12S391, Microsatellite polymorphism, SSCP analysis, STR, Sequence analysis, Suballeles",

author = "Y. Shigeta and Y. Yamamoto and Y. Doi and S. Miyaishi and H. Ishizu",

year = "1999",

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T1 - Polymorphism of the D12S391 microsatellite in a Japanese population sample

AU - Shigeta, Y.

AU - Yamamoto, Y.

AU - Doi, Y.

AU - Miyaishi, S.

AU - Ishizu, H.

PY - 1999/5/31

Y1 - 1999/5/31

N2 - Using the polymerase chain reaction (PCR), we studied the short tandem repeat (STR) polymorphism observed at the D12S391 locus. In 350 Japanese examined, 14 different alleles ranging from 209 bp to 261 bp were detected. Allele 18 (221 bp) showed the highest frequency at 0.30. Observed and expected values of respective genotypes satisfied the Hardy-Weinberg equilibrium (χ2 = 24.08, P = 0.24, df = 20). In addition, 18 additional sequence structures (suballeles), were detected in this study. Within the suballeles, sequence variants, in which the initial repeat of (AGAT) was replaced with (AGGT), was found in five samples. It was found that the analysis of single-strand conformation polymorphism (SSCP) before sequence analysis was useful for distinguishing these suballeles.

AB - Using the polymerase chain reaction (PCR), we studied the short tandem repeat (STR) polymorphism observed at the D12S391 locus. In 350 Japanese examined, 14 different alleles ranging from 209 bp to 261 bp were detected. Allele 18 (221 bp) showed the highest frequency at 0.30. Observed and expected values of respective genotypes satisfied the Hardy-Weinberg equilibrium (χ2 = 24.08, P = 0.24, df = 20). In addition, 18 additional sequence structures (suballeles), were detected in this study. Within the suballeles, sequence variants, in which the initial repeat of (AGAT) was replaced with (AGGT), was found in five samples. It was found that the analysis of single-strand conformation polymorphism (SSCP) before sequence analysis was useful for distinguishing these suballeles.

KW - D12S391

KW - Microsatellite polymorphism

KW - SSCP analysis

KW - STR

KW - Sequence analysis

KW - Suballeles

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JO - Forensic Science International

JF - Forensic Science International

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ER -

Polymorphism of the D12S391 microsatellite in a Japanese population sample

Abstract

Keywords

ASJC Scopus subject areas

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