Polymorphic microsatellite loci of the rat (Rattus norvegicus)

Tetsuo Kunieda, Eiji Kobayashi, Motokazu Tachibana, Hiroshi Ikadai, Tomonori Imamichi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The EMBL and GenBank DNA databases were searched for microsatellite sequences of the rat containing dinucleotide repeats of (CA)n and (GA)n. Among those obtained, 23 sequences were analyzed by polymerase chain reaction to examine the size variation of the amplified fragment in inbred rat strains. All of the 23 microsatellite sequences varied in size among the strains tested. The 23 microsatellite loci in a pair of substrains separated from the same progenitor strain were then analyzed. Fragments identical in size were observed in all loci of the two substrains, indicating the stability of the microsatellite over a large number of generations. The microsatellite loci, therefore, should be useful markers for linkage analyses in the rat.

Original languageEnglish
Pages (from-to)564-567
Number of pages4
JournalMammalian Genome
Volume3
Issue number10
DOIs
Publication statusPublished - Oct 1992
Externally publishedYes

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Microsatellite Repeats
Nucleic Acid Databases
Inbred Strains Rats
Dinucleotide Repeats
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics

Cite this

Kunieda, T., Kobayashi, E., Tachibana, M., Ikadai, H., & Imamichi, T. (1992). Polymorphic microsatellite loci of the rat (Rattus norvegicus). Mammalian Genome, 3(10), 564-567. https://doi.org/10.1007/BF00350622

Polymorphic microsatellite loci of the rat (Rattus norvegicus). / Kunieda, Tetsuo; Kobayashi, Eiji; Tachibana, Motokazu; Ikadai, Hiroshi; Imamichi, Tomonori.

In: Mammalian Genome, Vol. 3, No. 10, 10.1992, p. 564-567.

Research output: Contribution to journalArticle

Kunieda, T, Kobayashi, E, Tachibana, M, Ikadai, H & Imamichi, T 1992, 'Polymorphic microsatellite loci of the rat (Rattus norvegicus)', Mammalian Genome, vol. 3, no. 10, pp. 564-567. https://doi.org/10.1007/BF00350622
Kunieda, Tetsuo ; Kobayashi, Eiji ; Tachibana, Motokazu ; Ikadai, Hiroshi ; Imamichi, Tomonori. / Polymorphic microsatellite loci of the rat (Rattus norvegicus). In: Mammalian Genome. 1992 ; Vol. 3, No. 10. pp. 564-567.
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