Polymerase chain reaction with confronting two-pair primers for polymorphism genotyping

Nobuyuki Hamajima, Toshiko Saito, Keitaro Matsuo, Ken Ichi Kozaki, Takashi Takahashi, Kazuo Tajima

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

A novel PCR method using confronting two-pair primers, named PCR-CTPP, is introduced to detect a single nucleotide polymorphism (base X or Y). One primer for the X allele is set to include X' at the 3' end (antisense), where X' is the antisense of X, with the counterpart sense primer upstream. For the Y allele, a sense primer including Y at the 3' end is set, with the antisense primer downstream. One common band and one specific band for each allele are amplified, which allows genotyping directly by electrophoresis. This method is exemplified by application to the polymorphisms of beta-adrenoceptor 2 and interleukin 1B. It is simpler than PCR-RFLP (restriction fragment length polymorphism), which requires incubation with a restriction enzyme, and is suitable for genotyping in studies of genetic epidemiology involving hundreds of samples.

Original languageEnglish
Pages (from-to)865-868
Number of pages4
JournalJapanese Journal of Cancer Research
Volume91
Issue number9
DOIs
Publication statusPublished - 2000

Keywords

  • Confronting two-pair primers
  • PCR
  • Polymorphism

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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