Polymerase chain reaction-restriction fragment length polymorphism method for identifying carriers of hemophilia A in Japanese brown cattle

Maryam Khalaj, Abdol Rahim Abbasi, Takehito Tsuji, Yasuo Moritomo, Kenichi Shimojo, Tetsuo Kunieda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Hemophilia A is a severe congenital bleeding disorder characterized by subcutaneous hematoma and hemorrhage into muscles resulting from a deficiency of blood coagulation factor VIII. The authors have recently reported two cases of hemophilia A in Japanese Brown cattle and identified a nucleotide substitution in the factor VIII gene, resulting in an amino acid substitution of Leu to His, as a possible cause of the deficiency. In the present study, a simple and effective polymerase chain reaction (PCR)-based diagnostic method was developed to identify carriers of this disorder, using a mismatch primer in combination with restriction enzyme digestion. The PCR reaction amplified a 118 bp fragment, which was not digested by the BspT104I restriction enzyme in affected animals but was digested into two fragments in normal animals. Both digested and undigested fragments were observed in carrier animals. This method was applied to identify the carriers of hemophilia A in a population of Japanese Brown cattle. By screening 155 DNA samples from Japanese Brown cattle, except for the dam of the two probands, no carriers were identified. It was therefore concluded that the probands represent isolated cases of hemophilia A, and that the frequency of the mutant allele in the Japanese Brown cattle population is very low.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalAnimal Science Journal
Volume77
Issue number1
DOIs
Publication statusPublished - Feb 1 2006

Keywords

  • Bleeding disorder
  • Factor VIII
  • Hemophilia A
  • Japanese brown cattle
  • Polymerase chain reaction-restriction fragment length polymorphism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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