Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model

Hisako Fujimaki, Kentaro Uchida, Gen Inoue, Osamu Matsushita, Noriko Nemoto, Masayuki Miyagi, Kazuhide Inage, Shotaro Takano, Sumihisa Orita, Seiji Ohtori, Keisuke Tanaka, Hiroyuki Sekiguchi, Masashi Takaso

Research output: Contribution to journalArticle

Abstract

Several nerve conduits have been investigated for their potential as alternative sources of autografts for bridging neural gaps. However, autologous nerve transplants remain the most effective for nerve repair. We examined clinically approved nerve conduits containing collagen and polyglycolic acid (PGA-c) combined with collagen-binding basic fibroblast growth factor (bFGF) containing a polycystic kidney disease (PKD) domain and collagen binding domain (CBD) (bFGF-PKD-CBD) in a rat 15-mm sciatic nerve critical-size defect model. The treatment groups were: PGA-c immersed in phosphate-buffered saline (PBS) (PGA-c/PBS group), bFGF (PGA-c/bFGF group), or bFGF-PKD-CBD (PGA-c/bFGF-PKD-CBD group), and no treatment (Defect group). Gait and histological analyses were performed. Four weeks after treatment, the recovery rate of the paw print area was significantly greater in the PGA-c/bFGFPKD-CBD group than the PGA-c/PBS and PGA-c/bFGF groups. Mean intensity of paw prints was significantly greater in the PGA-c/bFGF-PKD-CBD group than the PGA-c/PBS and Defect groups. Swing time was significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. At 8 weeks, all three parameters were significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. Regenerated myelinated fibers were observed in 7/8 (87.5%) rats in the PGA-c/bFGF-PKD-CBD group after 8 weeks, and in 1/8 (12.5%) and 3/8 (37.5%) rats in the PGA-c/PBS and PGA-c/bFGF groups, respectively. PGA-c/bFGF-PKD-CBD composites may be promising biomaterials for promoting functional recovery of long-distance peripheral nerve defects in clinical practice.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Polyglycolic Acid
Fibroblast Growth Factor 2
Fibroblasts
Collagen
Rats
Recovery
Defects
Acids
Phosphates
Intercellular Signaling Peptides and Proteins

Keywords

  • basic fibroblast growth factor (bFGF)
  • collagen-binding domain
  • peripheral nerve regeneration
  • polyglycolic acid-collagen tube

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model. / Fujimaki, Hisako; Uchida, Kentaro; Inoue, Gen; Matsushita, Osamu; Nemoto, Noriko; Miyagi, Masayuki; Inage, Kazuhide; Takano, Shotaro; Orita, Sumihisa; Ohtori, Seiji; Tanaka, Keisuke; Sekiguchi, Hiroyuki; Takaso, Masashi.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 01.01.2019.

Research output: Contribution to journalArticle

Fujimaki, Hisako ; Uchida, Kentaro ; Inoue, Gen ; Matsushita, Osamu ; Nemoto, Noriko ; Miyagi, Masayuki ; Inage, Kazuhide ; Takano, Shotaro ; Orita, Sumihisa ; Ohtori, Seiji ; Tanaka, Keisuke ; Sekiguchi, Hiroyuki ; Takaso, Masashi. / Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2019.
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AU - Inoue, Gen

AU - Matsushita, Osamu

AU - Nemoto, Noriko

AU - Miyagi, Masayuki

AU - Inage, Kazuhide

AU - Takano, Shotaro

AU - Orita, Sumihisa

AU - Ohtori, Seiji

AU - Tanaka, Keisuke

AU - Sekiguchi, Hiroyuki

AU - Takaso, Masashi

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N2 - Several nerve conduits have been investigated for their potential as alternative sources of autografts for bridging neural gaps. However, autologous nerve transplants remain the most effective for nerve repair. We examined clinically approved nerve conduits containing collagen and polyglycolic acid (PGA-c) combined with collagen-binding basic fibroblast growth factor (bFGF) containing a polycystic kidney disease (PKD) domain and collagen binding domain (CBD) (bFGF-PKD-CBD) in a rat 15-mm sciatic nerve critical-size defect model. The treatment groups were: PGA-c immersed in phosphate-buffered saline (PBS) (PGA-c/PBS group), bFGF (PGA-c/bFGF group), or bFGF-PKD-CBD (PGA-c/bFGF-PKD-CBD group), and no treatment (Defect group). Gait and histological analyses were performed. Four weeks after treatment, the recovery rate of the paw print area was significantly greater in the PGA-c/bFGFPKD-CBD group than the PGA-c/PBS and PGA-c/bFGF groups. Mean intensity of paw prints was significantly greater in the PGA-c/bFGF-PKD-CBD group than the PGA-c/PBS and Defect groups. Swing time was significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. At 8 weeks, all three parameters were significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. Regenerated myelinated fibers were observed in 7/8 (87.5%) rats in the PGA-c/bFGF-PKD-CBD group after 8 weeks, and in 1/8 (12.5%) and 3/8 (37.5%) rats in the PGA-c/PBS and PGA-c/bFGF groups, respectively. PGA-c/bFGF-PKD-CBD composites may be promising biomaterials for promoting functional recovery of long-distance peripheral nerve defects in clinical practice.

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