Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model

Hisako Fujimaki, Kentaro Uchida, Gen Inoue, Osamu Matsushita, Noriko Nemoto, Masayuki Miyagi, Kazuhide Inage, Shotaro Takano, Sumihisa Orita, Seiji Ohtori, Keisuke Tanaka, Hiroyuki Sekiguchi, Masashi Takaso

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Several nerve conduits have been investigated for their potential as alternative sources of autografts for bridging neural gaps. However, autologous nerve transplants remain the most effective for nerve repair. We examined clinically approved nerve conduits containing collagen and polyglycolic acid (PGA-c) combined with collagen-binding basic fibroblast growth factor (bFGF) containing a polycystic kidney disease (PKD) domain and collagen binding domain (CBD) (bFGF-PKD-CBD) in a rat 15-mm sciatic nerve critical-size defect model. The treatment groups were: PGA-c immersed in phosphate-buffered saline (PBS) (PGA-c/PBS group), bFGF (PGA-c/bFGF group), or bFGF-PKD-CBD (PGA-c/bFGF-PKD-CBD group), and no treatment (Defect group). Gait and histological analyses were performed. Four weeks after treatment, the recovery rate of the paw print area was significantly greater in the PGA-c/bFGFPKD-CBD group than the PGA-c/PBS and PGA-c/bFGF groups. Mean intensity of paw prints was significantly greater in the PGA-c/bFGF-PKD-CBD group than the PGA-c/PBS and Defect groups. Swing time was significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. At 8 weeks, all three parameters were significantly greater in the PGA-c/PBS, PGA-c/bFGF, and PGA-c/bFGF-PKD-CBD groups than the Defect group. Regenerated myelinated fibers were observed in 7/8 (87.5%) rats in the PGA-c/bFGF-PKD-CBD group after 8 weeks, and in 1/8 (12.5%) and 3/8 (37.5%) rats in the PGA-c/PBS and PGA-c/bFGF groups, respectively. PGA-c/bFGF-PKD-CBD composites may be promising biomaterials for promoting functional recovery of long-distance peripheral nerve defects in clinical practice.

Original languageEnglish
Pages (from-to)326-332
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume108
Issue number2
DOIs
Publication statusPublished - Feb 1 2020
Externally publishedYes

Keywords

  • basic fibroblast growth factor (bFGF)
  • collagen-binding domain
  • peripheral nerve regeneration
  • polyglycolic acid-collagen tube

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Fingerprint Dive into the research topics of 'Polyglycolic acid-collagen tube combined with collagen-binding basic fibroblast growth factor accelerates gait recovery in a rat sciatic nerve critical-size defect model'. Together they form a unique fingerprint.

  • Cite this