Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

Akiwo Tani, Peechapack Somyoonsap, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalArchives of Microbiology
Volume189
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Coenzyme A
Ligases
Metabolism
Coenzyme A Ligases
Glycolaldehyde Dehydrogenase
Periplasm
Detoxification
Operon
Acidity
Cytoplasm
Fatty Acids
Cell Membrane
Genes
Membranes
Degradation
Substrates
Proteins

Keywords

  • Acyl-CoA synthetase
  • Peg operon
  • Polyethylene glycol metabolism
  • Sphingopyxis macrogoltabida

ASJC Scopus subject areas

  • Microbiology

Cite this

Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103. / Tani, Akiwo; Somyoonsap, Peechapack; Minami, Toshiyuki; Kimbara, Kazuhide; Kawai, Fusako.

In: Archives of Microbiology, Vol. 189, No. 4, 04.2008, p. 407-410.

Research output: Contribution to journalArticle

Tani, Akiwo ; Somyoonsap, Peechapack ; Minami, Toshiyuki ; Kimbara, Kazuhide ; Kawai, Fusako. / Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103. In: Archives of Microbiology. 2008 ; Vol. 189, No. 4. pp. 407-410.
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