Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

Akio Tani, Peechapack Somyoonsap, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

Original languageEnglish
Pages (from-to)407-410
Number of pages4
JournalArchives of Microbiology
Volume189
Issue number4
DOIs
Publication statusPublished - Apr 1 2008

Keywords

  • Acyl-CoA synthetase
  • Peg operon
  • Polyethylene glycol metabolism
  • Sphingopyxis macrogoltabida

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103'. Together they form a unique fingerprint.

  • Cite this