Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

Akio Tani; Peechapack Somyoonsap; Toshiyuki Minami; Kazuhide Kimbara; Fusako Kawai

doi:10.1007/s00203-007-0320-z

Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

Akio Tani, Peechapack Somyoonsap, Toshiyuki Minami, Kazuhide Kimbara, Fusako Kawai

Institute of Plant Science and Resources

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

Original language	English
Pages (from-to)	407-410
Number of pages	4
Journal	Archives of Microbiology
Volume	189
Issue number	4
DOIs	https://doi.org/10.1007/s00203-007-0320-z
Publication status	Published - Apr 2008

Keywords

Acyl-CoA synthetase
Peg operon
Polyethylene glycol metabolism
Sphingopyxis macrogoltabida

ASJC Scopus subject areas

Microbiology
Biochemistry
Molecular Biology
Genetics

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10.1007/s00203-007-0320-z

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title = "Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103",

abstract = "Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.",

keywords = "Acyl-CoA synthetase, Peg operon, Polyethylene glycol metabolism, Sphingopyxis macrogoltabida",

author = "Akio Tani and Peechapack Somyoonsap and Toshiyuki Minami and Kazuhide Kimbara and Fusako Kawai",

note = "Funding Information: Acknowledgment This work was partly supported by Wesco Scien-tiWc Promotion Foundation and GRANT-IN-AID FOR SCIENTIFIC RESEARCH (C) 19780060 from the Japan Society for the Promotion of Science.",

year = "2008",

month = apr,

doi = "10.1007/s00203-007-0320-z",

language = "English",

volume = "189",

pages = "407--410",

journal = "Archives of Microbiology",

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T1 - Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

AU - Tani, Akio

AU - Somyoonsap, Peechapack

AU - Minami, Toshiyuki

AU - Kimbara, Kazuhide

AU - Kawai, Fusako

N1 - Funding Information: Acknowledgment This work was partly supported by Wesco Scien-tiWc Promotion Foundation and GRANT-IN-AID FOR SCIENTIFIC RESEARCH (C) 19780060 from the Japan Society for the Promotion of Science.

PY - 2008/4

Y1 - 2008/4

N2 - Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

AB - Sphingopyxis macrogoltabida strain 103 possesses polyethylene-glycol (PEG)-inducible pegBCDAE operon encoding the genes relevant to PEG degradation. PEG is converted to PEG-carboxylate by PegA (PEG dehydrogenase) and PegC (PEG-aldehyde dehydrogenase). In this study, the recombinant PegE (homologous to acyl-CoA synthetases) was characterized. PegE was an acyl-CoA synthetase active for PEG-carboxylate and fatty acids. Judging from the nature of this kind of protein (located on the cytoplasmic membrane as a translocator), PegE might be responsible for the translocation of PEG-carboxylate from the periplasm into the cytoplasm or for the detoxification of strong acidity of the substrate.

KW - Acyl-CoA synthetase

KW - Peg operon

KW - Polyethylene glycol metabolism

KW - Sphingopyxis macrogoltabida

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SN - 0302-8933

VL - 189

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EP - 410

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 4

ER -

Polyethylene glycol (PEG)-carboxylate-CoA synthetase is involved in PEG metabolism in Sphingopyxis macrogoltabida strain 103

Abstract

Keywords

ASJC Scopus subject areas

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