Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells

Nobuhiro Suzuki, Mikiko Sugawara, Donald L. Nuss, Yoshiharu Matsuura

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Genomic segment S12 of rice dwarf virus and segment S9 of wound tumor virus, both members of the genus Phytoreovirus, have small out-of-phase overlapping open reading frames (ORFs). Western blot (immunoblot) analysis revealed that rice dwarf virus S12 mRNA specified translation products from the large ORF and two overlapping small ORFs both in rice plant hosts and in Spodoptera frugiperda insect cells. These results provide the first example of a tricistronic mRNA for a segmented double-stranded RNA virus. Similarly, wound tumor virus S9 mRNA was found to direct the synthesis of protein products from both the large ORF and small out-of-frame ORF in S. frugiperda cells. Results of site-specific and deletion mutagenesis studies were consistent with a leaky scanning translation mechanism for the synthesis of the small ORFs.

Original languageEnglish
Pages (from-to)8155-8159
Number of pages5
JournalJournal of Virology
Volume70
Issue number11
Publication statusPublished - Nov 1996
Externally publishedYes

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Plant Cells
Open Reading Frames
Insects
open reading frames
Wound tumor virus
insects
Rice dwarf virus
Spodoptera
Oncogenic Viruses
Spodoptera frugiperda
cells
translation (genetics)
Phytoreovirus
Western Blotting
Reoviridae
dsRNA viruses
Viruses
Messenger RNA
synthesis
Double-Stranded RNA

ASJC Scopus subject areas

  • Immunology

Cite this

Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells. / Suzuki, Nobuhiro; Sugawara, Mikiko; Nuss, Donald L.; Matsuura, Yoshiharu.

In: Journal of Virology, Vol. 70, No. 11, 11.1996, p. 8155-8159.

Research output: Contribution to journalArticle

Suzuki, Nobuhiro ; Sugawara, Mikiko ; Nuss, Donald L. ; Matsuura, Yoshiharu. / Polycistronic (tri- or bicistronic) phytoreoviral segments translatable in both plant and insect cells. In: Journal of Virology. 1996 ; Vol. 70, No. 11. pp. 8155-8159.
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