Polyamine and differentiation: Induction of ornithine decarboxylase by parathyroid hormone is a good marker of differentiated chondrocytes

Masaharu Takigawa, H. Ishida, T. Takano, F. Suzuki

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115 Citations (Scopus)

Abstract

The activity of ornithine decarboxylase (OD-Case; L-ornithine carboxy-lyase, EC 4.1.1.17) in rabbit costal chondrocytes in culture increased markedly after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 hr after PTH addition. The increase in ODCase activity was followed by increase in the intracellular concentrations of polyamines, especially putrescine, which increased in 6 hr to about 3-fold that of untreated cultures. The induction of ODCase by PTH was not observed in L, 3T3, HeLa, buffalo rat liver, or BHK cells. Retinyl acetate and retinoic acid both inhibited expression of the differentiated phenotype of chondrocytes by rabbit costal chondrocytes in culture within 3 days after their addition, as judged by morphological change and decrease in sulfate incorporation into glycosaminoglycans but did not inhibit cell proliferation. PTH could not induce an increase in ODCase in de-differentiated cells that had been pretreated with retinyl acetate or retinoic acid for 3 days; but 4 days after removal of the retinoids, these de-differentiated cells regained the ability to synthesize ODCase in response to PTH. These facts suggest that the induction of ODCase and the formation of putrescine by PTH are good markers of the differentiated phenotype of cultured chondrocytes.

Original languageEnglish
Pages (from-to)1481-1485
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number3 I
Publication statusPublished - 1980
Externally publishedYes

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Ornithine Decarboxylase
Polyamines
Chondrocytes
Parathyroid Hormone
Putrescine
Tretinoin
Rabbits
Phenotype
Buffaloes
Retinoids
Glycosaminoglycans
Sulfates
Cell Proliferation
Liver

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Polyamine and differentiation: Induction of ornithine decarboxylase by parathyroid hormone is a good marker of differentiated chondrocytes",
abstract = "The activity of ornithine decarboxylase (OD-Case; L-ornithine carboxy-lyase, EC 4.1.1.17) in rabbit costal chondrocytes in culture increased markedly after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 hr after PTH addition. The increase in ODCase activity was followed by increase in the intracellular concentrations of polyamines, especially putrescine, which increased in 6 hr to about 3-fold that of untreated cultures. The induction of ODCase by PTH was not observed in L, 3T3, HeLa, buffalo rat liver, or BHK cells. Retinyl acetate and retinoic acid both inhibited expression of the differentiated phenotype of chondrocytes by rabbit costal chondrocytes in culture within 3 days after their addition, as judged by morphological change and decrease in sulfate incorporation into glycosaminoglycans but did not inhibit cell proliferation. PTH could not induce an increase in ODCase in de-differentiated cells that had been pretreated with retinyl acetate or retinoic acid for 3 days; but 4 days after removal of the retinoids, these de-differentiated cells regained the ability to synthesize ODCase in response to PTH. These facts suggest that the induction of ODCase and the formation of putrescine by PTH are good markers of the differentiated phenotype of cultured chondrocytes.",
author = "Masaharu Takigawa and H. Ishida and T. Takano and F. Suzuki",
year = "1980",
language = "English",
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T2 - Induction of ornithine decarboxylase by parathyroid hormone is a good marker of differentiated chondrocytes

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AU - Ishida, H.

AU - Takano, T.

AU - Suzuki, F.

PY - 1980

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AB - The activity of ornithine decarboxylase (OD-Case; L-ornithine carboxy-lyase, EC 4.1.1.17) in rabbit costal chondrocytes in culture increased markedly after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 hr after PTH addition. The increase in ODCase activity was followed by increase in the intracellular concentrations of polyamines, especially putrescine, which increased in 6 hr to about 3-fold that of untreated cultures. The induction of ODCase by PTH was not observed in L, 3T3, HeLa, buffalo rat liver, or BHK cells. Retinyl acetate and retinoic acid both inhibited expression of the differentiated phenotype of chondrocytes by rabbit costal chondrocytes in culture within 3 days after their addition, as judged by morphological change and decrease in sulfate incorporation into glycosaminoglycans but did not inhibit cell proliferation. PTH could not induce an increase in ODCase in de-differentiated cells that had been pretreated with retinyl acetate or retinoic acid for 3 days; but 4 days after removal of the retinoids, these de-differentiated cells regained the ability to synthesize ODCase in response to PTH. These facts suggest that the induction of ODCase and the formation of putrescine by PTH are good markers of the differentiated phenotype of cultured chondrocytes.

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