Abstract
We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.
| Original language | English |
|---|---|
| Pages (from-to) | 45-48 |
| Number of pages | 4 |
| Journal | Journal of Neuroscience Methods |
| Volume | 73 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Apr 25 1997 |
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Keywords
- 6-Hydroxydopamine
- Amine concentration
- Degeneration of nigrostriatal dopamine neurons
- Locomotor activity time
- Mouse movement disorder
- Parkinson's disease
- Pole test
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion. / Matsuura, Kouji; Kabuto, Hideaki; Makino, Hirofumi; Ogawa, Norio.
In: Journal of Neuroscience Methods, Vol. 73, No. 1, 25.04.1997, p. 45-48.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion
AU - Matsuura, Kouji
AU - Kabuto, Hideaki
AU - Makino, Hirofumi
AU - Ogawa, Norio
PY - 1997/4/25
Y1 - 1997/4/25
N2 - We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.
AB - We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.
KW - 6-Hydroxydopamine
KW - Amine concentration
KW - Degeneration of nigrostriatal dopamine neurons
KW - Locomotor activity time
KW - Mouse movement disorder
KW - Parkinson's disease
KW - Pole test
UR - http://www.scopus.com/inward/record.url?scp=0343052652&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0343052652&partnerID=8YFLogxK
U2 - 10.1016/S0165-0270(96)02211-X
DO - 10.1016/S0165-0270(96)02211-X
M3 - Article
VL - 73
SP - 45
EP - 48
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1
ER -