Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion

Kouji Matsuura, Hideaki Kabuto, Hirojumi Makino, Norio Ogawa

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalJournal of Neuroscience Methods
Volume73
Issue number1
DOIs
Publication statusPublished - Apr 25 1997

Keywords

  • 6-Hydroxydopamine
  • Amine concentration
  • Degeneration of nigrostriatal dopamine neurons
  • Locomotor activity time
  • Mouse movement disorder
  • Parkinson's disease
  • Pole test

ASJC Scopus subject areas

  • Neuroscience(all)

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