Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion

Kouji Matsuura, Hideaki Kabuto, Hirofumi Makino, Norio Ogawa

Research output: Contribution to journalArticle

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Abstract

We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalJournal of Neuroscience Methods
Volume73
Issue number1
DOIs
Publication statusPublished - Apr 25 1997

Fingerprint

Corpus Striatum
Oxidopamine
Movement Disorders
Dopamine
Injections
Serotonin
Homovanillic Acid
Hydroxyindoleacetic Acid
Locomotion
Animal Models
Acids

Keywords

  • 6-Hydroxydopamine
  • Amine concentration
  • Degeneration of nigrostriatal dopamine neurons
  • Locomotor activity time
  • Mouse movement disorder
  • Parkinson's disease
  • Pole test

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion. / Matsuura, Kouji; Kabuto, Hideaki; Makino, Hirofumi; Ogawa, Norio.

In: Journal of Neuroscience Methods, Vol. 73, No. 1, 25.04.1997, p. 45-48.

Research output: Contribution to journalArticle

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