TY - JOUR
T1 - Pole test is a useful method for evaluating the mouse movement disorder caused by striatal dopamine depletion
AU - Matsuura, Kouji
AU - Kabuto, Hideaki
AU - Makino, Hirojumi
AU - Ogawa, Norio
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture, and by a Grant-in-Aid from the Research Committee on CNS degenerative diseases of the Japanese Ministry of Health and Welfare.
PY - 1997/4/25
Y1 - 1997/4/25
N2 - We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.
AB - We evaluated the behavioral recovery of mice with 6-hydroxydopamine (6-OHDA)-induced lesions using a pole test. T(LA) (locomotor activity time) 1, 2, and 3 days after intracerebroventricular 6-OHDA injection (T(LA(1-3D)) was correlated significantly with the levels of-dopamine (DA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the striatum 7 days after the injection of 6-OHDA, but 5-hydroxyindoleacetic acid (5-HIAA) and serotonin (5-HT) had no correlation with T(LA(1-3D)). The mice whose T(LA(1-3D)) was more than the median showed about 60% depletion of striatal DA and increased DA turnover, and recovered from movement disorders 4 days after injection. These results show that presynaptic neuroadaptations and behavioral recovery exist in this animal model. Thus, the pole test appears to be useful in predicting the extent of the lesion to select a mouse in which the receptive fields of the dopaminergic cells are denervated.
KW - 6-Hydroxydopamine
KW - Amine concentration
KW - Degeneration of nigrostriatal dopamine neurons
KW - Locomotor activity time
KW - Mouse movement disorder
KW - Parkinson's disease
KW - Pole test
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U2 - 10.1016/S0165-0270(96)02211-X
DO - 10.1016/S0165-0270(96)02211-X
M3 - Article
C2 - 9130677
AN - SCOPUS:0343052652
VL - 73
SP - 45
EP - 48
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
SN - 0165-0270
IS - 1
ER -