Point mutations in the S and pre-S2 genes observed in two hepatitis B virus carriers positive for antibody to hepatitis B surface antigen

Toshiyuki Shinji, Norio Koide, Tadashi Hanafusa, Hajime Hada, Takahiko Oka, Niro Takayama, Hidenori Shiraha, Masaki Nakamura, Kozo Ujike, Yasuhiro Yumoto, Takao Tsuji

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Two hepatitis B virus (HBV) carriers who had antibodies to HBV surface antigen (anti-HBs) were studied. Case 1 was a 47 year old woman positive for hepatitis B e antigen (HBeAg), and case 2 was a 61 year old man positive for antibody to HBeAg (anti-HBe) and DNA-polymerase (DNA-p). Neither case had received the HBV vaccine. The nucleotide sequences of the HBV-DNA extracted from the patient's sera were determined within the pre-S2 and S genes. Seven out of nine S gene clones from case 1 and six out of nine S gene clones from case 2 had an amino acid replacement from Thr of Ile to Ser at codon 126 in the α-determinant of the S gene. Amino acid substitution of codon 145 of the S gene previously reported was not observed. Although two previous reports of HBV escape mutant carriers with both anti-HBs and HBeAg described some deletions in the pre-S2 gene, our cased did not show these deletions. Our analysis indicated that carriers with the HBV escape mutant did not always have pre-S2 gene deletions. We found two HBV escape mutant carriers who had amino acid substitutions at codon 126 in the S gene due to point mutation without any deletions in the pre-S2 gene.

Original languageEnglish
Pages (from-to)500-502
Number of pages3
JournalHepato-gastroenterology
Volume45
Issue number20
Publication statusPublished - Apr 27 1998

Keywords

  • HBV carrier
  • HBV escape mutant
  • Pre-S2 gene
  • S gene

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Point mutations in the S and pre-S2 genes observed in two hepatitis B virus carriers positive for antibody to hepatitis B surface antigen'. Together they form a unique fingerprint.

  • Cite this

    Shinji, T., Koide, N., Hanafusa, T., Hada, H., Oka, T., Takayama, N., Shiraha, H., Nakamura, M., Ujike, K., Yumoto, Y., & Tsuji, T. (1998). Point mutations in the S and pre-S2 genes observed in two hepatitis B virus carriers positive for antibody to hepatitis B surface antigen. Hepato-gastroenterology, 45(20), 500-502.