PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice

Keiki Ogino, Kenjiro Nagaoka, Tomoaki Okuda, Akira Oka, Masayuki Kubo, Eri Eguchi, Yoshihisa Fujikura

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The allergic inflammatory effects of particulate matter (PM) 2.5, collected with the cyclone system in Yokohama city in Japan, were investigated in NC/Nga mice, which are hypersensitive to mite allergens. PM2.5 with alum was injected intraperitoneally for sensitization. Five days later, 200 μg of PM2.5 in 25 μL of saline was administered to mice intranasally five times for further sensitization. On the 11th day, PM2.5 was administered as a challenge. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), the bronchoalveolar lavage fluid (BALF) cell count, mRNA expression of Th1, Th2 cytokines, and metallothioneins in lung tissue, and histopathology. PM2.5 increased AHR, total cell numbers including eosinophils in BALF, and mRNA levels of IL-5, IL-22, eotaxin, eotaxin 2, and metallothionein 3. In PM2.5-induced lungs, inflammation was observed around the bronchus. These results demonstrate that PM2.5 alone, collected with the cyclone system in Yokohama city in Japan, induces asthma-like airway inflammation.

Original languageEnglish
Pages (from-to)1047-1054
Number of pages8
JournalEnvironmental Toxicology
Volume32
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

cyclone
Cyclonic Storms
Bronchoalveolar Lavage Fluid
Inflammation
histopathology
metallothionein
fluid
asthma
Chemokine CCL24
Japan
Cell Count
mite
particulate matter
Messenger RNA
Fluids
Particulate Matter
Metallothionein
Mites
Interleukin-5
Bronchi

Keywords

  • allergic airway inflammation
  • NC/Nga mice
  • PM2.5

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Ogino, K., Nagaoka, K., Okuda, T., Oka, A., Kubo, M., Eguchi, E., & Fujikura, Y. (2017). PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice. Environmental Toxicology, 32(3), 1047-1054. https://doi.org/10.1002/tox.22303

PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice. / Ogino, Keiki; Nagaoka, Kenjiro; Okuda, Tomoaki; Oka, Akira; Kubo, Masayuki; Eguchi, Eri; Fujikura, Yoshihisa.

In: Environmental Toxicology, Vol. 32, No. 3, 01.03.2017, p. 1047-1054.

Research output: Contribution to journalArticle

Ogino, K, Nagaoka, K, Okuda, T, Oka, A, Kubo, M, Eguchi, E & Fujikura, Y 2017, 'PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice', Environmental Toxicology, vol. 32, no. 3, pp. 1047-1054. https://doi.org/10.1002/tox.22303
Ogino, Keiki ; Nagaoka, Kenjiro ; Okuda, Tomoaki ; Oka, Akira ; Kubo, Masayuki ; Eguchi, Eri ; Fujikura, Yoshihisa. / PM2.5-induced airway inflammation and hyperresponsiveness in NC/Nga mice. In: Environmental Toxicology. 2017 ; Vol. 32, No. 3. pp. 1047-1054.
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