Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing

Yoshiteru Kajikawa, Toru Morihara, Hirotaka Sakamoto, Ken Ichi Matsuda, Yasushi Oshima, Atsuhiko Yoshida, Masateru Nagae, Yuji Arai, Mitsuhiro Kawata, Toshikazu Kubo

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

Circulation-derived cells play a crucial role in the healing processes of tissue. In early phases of tendon healing processes, circulation-derived cells temporarily exist in the wounded area to initiate the healing process and decrease in number with time. We assumed that a delay of time-dependent decrease in circulation-derived cells could improve the healing of tendons. In this study, we injected platelet-rich plasma (PRP) containing various kinds of growth factors into the wounded area of the patellar tendon, and compared the effects on activation of circulation-derived cells and enhancement of tendon healing with a control group (no PRP injection). To follow the circulation-derived cells, we used a green fluorescent protein (GFP) chimeric rat expressing GFP in the circulating cells and bone marrow cells. In the PRP group, the numbers of GFP-positive cells and heat-shock protein (HSP47; collagen-specific molecular chaperone)-positive cells were significantly higher than in the control group at 3 and 7 days after injury. At the same time, the immunoreactivity for types I and III collagen was higher in the PRP group than in the control group at early phase of tendon healing. These findings suggest that locally injected PRP is useful as an activator of circulation-derived cells for enhancement of the initial tendon healing process.

Original languageEnglish
Pages (from-to)837-845
Number of pages9
JournalJournal of Cellular Physiology
Volume215
Issue number3
DOIs
Publication statusPublished - Jun 2008
Externally publishedYes

Fingerprint

Platelet-Rich Plasma
Tendons
Platelets
Plasmas
Green Fluorescent Proteins
Cells
HSP47 Heat-Shock Proteins
Control Groups
Molecular Chaperones
Collagen Type III
Collagen Type I
Rats
Patellar Ligament
Intercellular Signaling Peptides and Proteins
Bone
Collagen
Chemical activation
Tissue
Bone Marrow Cells
Injections

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. / Kajikawa, Yoshiteru; Morihara, Toru; Sakamoto, Hirotaka; Matsuda, Ken Ichi; Oshima, Yasushi; Yoshida, Atsuhiko; Nagae, Masateru; Arai, Yuji; Kawata, Mitsuhiro; Kubo, Toshikazu.

In: Journal of Cellular Physiology, Vol. 215, No. 3, 06.2008, p. 837-845.

Research output: Contribution to journalArticle

Kajikawa, Y, Morihara, T, Sakamoto, H, Matsuda, KI, Oshima, Y, Yoshida, A, Nagae, M, Arai, Y, Kawata, M & Kubo, T 2008, 'Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing', Journal of Cellular Physiology, vol. 215, no. 3, pp. 837-845. https://doi.org/10.1002/jcp.21368
Kajikawa, Yoshiteru ; Morihara, Toru ; Sakamoto, Hirotaka ; Matsuda, Ken Ichi ; Oshima, Yasushi ; Yoshida, Atsuhiko ; Nagae, Masateru ; Arai, Yuji ; Kawata, Mitsuhiro ; Kubo, Toshikazu. / Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing. In: Journal of Cellular Physiology. 2008 ; Vol. 215, No. 3. pp. 837-845.
@article{ebd32dfd5dae487da241020a8f252858,
title = "Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing",
abstract = "Circulation-derived cells play a crucial role in the healing processes of tissue. In early phases of tendon healing processes, circulation-derived cells temporarily exist in the wounded area to initiate the healing process and decrease in number with time. We assumed that a delay of time-dependent decrease in circulation-derived cells could improve the healing of tendons. In this study, we injected platelet-rich plasma (PRP) containing various kinds of growth factors into the wounded area of the patellar tendon, and compared the effects on activation of circulation-derived cells and enhancement of tendon healing with a control group (no PRP injection). To follow the circulation-derived cells, we used a green fluorescent protein (GFP) chimeric rat expressing GFP in the circulating cells and bone marrow cells. In the PRP group, the numbers of GFP-positive cells and heat-shock protein (HSP47; collagen-specific molecular chaperone)-positive cells were significantly higher than in the control group at 3 and 7 days after injury. At the same time, the immunoreactivity for types I and III collagen was higher in the PRP group than in the control group at early phase of tendon healing. These findings suggest that locally injected PRP is useful as an activator of circulation-derived cells for enhancement of the initial tendon healing process.",
author = "Yoshiteru Kajikawa and Toru Morihara and Hirotaka Sakamoto and Matsuda, {Ken Ichi} and Yasushi Oshima and Atsuhiko Yoshida and Masateru Nagae and Yuji Arai and Mitsuhiro Kawata and Toshikazu Kubo",
year = "2008",
month = "6",
doi = "10.1002/jcp.21368",
language = "English",
volume = "215",
pages = "837--845",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "3",

}

TY - JOUR

T1 - Platelet-rich plasma enhances the initial mobilization of circulation-derived cells for tendon healing

AU - Kajikawa, Yoshiteru

AU - Morihara, Toru

AU - Sakamoto, Hirotaka

AU - Matsuda, Ken Ichi

AU - Oshima, Yasushi

AU - Yoshida, Atsuhiko

AU - Nagae, Masateru

AU - Arai, Yuji

AU - Kawata, Mitsuhiro

AU - Kubo, Toshikazu

PY - 2008/6

Y1 - 2008/6

N2 - Circulation-derived cells play a crucial role in the healing processes of tissue. In early phases of tendon healing processes, circulation-derived cells temporarily exist in the wounded area to initiate the healing process and decrease in number with time. We assumed that a delay of time-dependent decrease in circulation-derived cells could improve the healing of tendons. In this study, we injected platelet-rich plasma (PRP) containing various kinds of growth factors into the wounded area of the patellar tendon, and compared the effects on activation of circulation-derived cells and enhancement of tendon healing with a control group (no PRP injection). To follow the circulation-derived cells, we used a green fluorescent protein (GFP) chimeric rat expressing GFP in the circulating cells and bone marrow cells. In the PRP group, the numbers of GFP-positive cells and heat-shock protein (HSP47; collagen-specific molecular chaperone)-positive cells were significantly higher than in the control group at 3 and 7 days after injury. At the same time, the immunoreactivity for types I and III collagen was higher in the PRP group than in the control group at early phase of tendon healing. These findings suggest that locally injected PRP is useful as an activator of circulation-derived cells for enhancement of the initial tendon healing process.

AB - Circulation-derived cells play a crucial role in the healing processes of tissue. In early phases of tendon healing processes, circulation-derived cells temporarily exist in the wounded area to initiate the healing process and decrease in number with time. We assumed that a delay of time-dependent decrease in circulation-derived cells could improve the healing of tendons. In this study, we injected platelet-rich plasma (PRP) containing various kinds of growth factors into the wounded area of the patellar tendon, and compared the effects on activation of circulation-derived cells and enhancement of tendon healing with a control group (no PRP injection). To follow the circulation-derived cells, we used a green fluorescent protein (GFP) chimeric rat expressing GFP in the circulating cells and bone marrow cells. In the PRP group, the numbers of GFP-positive cells and heat-shock protein (HSP47; collagen-specific molecular chaperone)-positive cells were significantly higher than in the control group at 3 and 7 days after injury. At the same time, the immunoreactivity for types I and III collagen was higher in the PRP group than in the control group at early phase of tendon healing. These findings suggest that locally injected PRP is useful as an activator of circulation-derived cells for enhancement of the initial tendon healing process.

UR - http://www.scopus.com/inward/record.url?scp=42949147032&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42949147032&partnerID=8YFLogxK

U2 - 10.1002/jcp.21368

DO - 10.1002/jcp.21368

M3 - Article

C2 - 18181148

AN - SCOPUS:42949147032

VL - 215

SP - 837

EP - 845

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 3

ER -