Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140)

S. J. Israels, J. M. Gerrard, Y. V. Jacques, A. McNicol, B. Cham, Masahiro Nishibori, D. F. Bainton

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

We recently reported the characterization of a platelet granule membrane protein of molecular weight (mol wt) 40,000 called granulophysin (Gerrard et al: Blood 77:101, 1991), identified by a monoclonal antibody (MoAb D545) raised to purified dense granule membranes. Using immunoelectron-microscopic techniques on frozen thin sections, this protein was localized in resting and thrombin-stimulated platelets. In resting platelets, labeled with antigranulophysin antibodies and immunogold probes, label was localized to the membranes of one or two clear granules per platelet thin section. D545 also labeled dense granules in permeabilized whole platelets and isolated dense granule preparations examined by whole-mount techniques. Expression of granulophysin on the platelet surface paralleled dense granule secretion as measured by 14C-serotonin release under conditions in which lysosomal granule release, as measured by β-glucuronidase secretion, was less than 5%. After thrombin stimulation, both the surface-connected canalicular system and the plasma membrane were labeled, demonstrating redistribution of granulophysin associated with degranulation. Double labeling experiments with D545 and antibodies to the α-granule membrane protein, P-selectin, demonstrated labeling of both P-selectin and granulophysin on dense granule membranes. Distribution of both proteins on the plasma membrane after platelet stimulation was similar. The results demonstrate that granulophysin is localized to the dense granules of platelets and is redistributed to the plasma membrane after platelet activation.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalBlood
Volume80
Issue number1
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

CD63 Antigen
P-Selectin
Platelets
Blood Platelets
Membranes
Cell membranes
Cell Membrane
Thrombin
Labeling
Membrane Proteins
Antibodies
Glucuronidase
Platelet Activation
Frozen Sections
Serotonin
Proteins
Labels
Molecular Weight
Monoclonal Antibodies

ASJC Scopus subject areas

  • Hematology

Cite this

Israels, S. J., Gerrard, J. M., Jacques, Y. V., McNicol, A., Cham, B., Nishibori, M., & Bainton, D. F. (1992). Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). Blood, 80(1), 143-152.

Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). / Israels, S. J.; Gerrard, J. M.; Jacques, Y. V.; McNicol, A.; Cham, B.; Nishibori, Masahiro; Bainton, D. F.

In: Blood, Vol. 80, No. 1, 1992, p. 143-152.

Research output: Contribution to journalArticle

Israels, SJ, Gerrard, JM, Jacques, YV, McNicol, A, Cham, B, Nishibori, M & Bainton, DF 1992, 'Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140)', Blood, vol. 80, no. 1, pp. 143-152.
Israels SJ, Gerrard JM, Jacques YV, McNicol A, Cham B, Nishibori M et al. Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). Blood. 1992;80(1):143-152.
Israels, S. J. ; Gerrard, J. M. ; Jacques, Y. V. ; McNicol, A. ; Cham, B. ; Nishibori, Masahiro ; Bainton, D. F. / Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140). In: Blood. 1992 ; Vol. 80, No. 1. pp. 143-152.
@article{e1d68b7e778e4f838df0e3b36fea4376,
title = "Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140)",
abstract = "We recently reported the characterization of a platelet granule membrane protein of molecular weight (mol wt) 40,000 called granulophysin (Gerrard et al: Blood 77:101, 1991), identified by a monoclonal antibody (MoAb D545) raised to purified dense granule membranes. Using immunoelectron-microscopic techniques on frozen thin sections, this protein was localized in resting and thrombin-stimulated platelets. In resting platelets, labeled with antigranulophysin antibodies and immunogold probes, label was localized to the membranes of one or two clear granules per platelet thin section. D545 also labeled dense granules in permeabilized whole platelets and isolated dense granule preparations examined by whole-mount techniques. Expression of granulophysin on the platelet surface paralleled dense granule secretion as measured by 14C-serotonin release under conditions in which lysosomal granule release, as measured by β-glucuronidase secretion, was less than 5{\%}. After thrombin stimulation, both the surface-connected canalicular system and the plasma membrane were labeled, demonstrating redistribution of granulophysin associated with degranulation. Double labeling experiments with D545 and antibodies to the α-granule membrane protein, P-selectin, demonstrated labeling of both P-selectin and granulophysin on dense granule membranes. Distribution of both proteins on the plasma membrane after platelet stimulation was similar. The results demonstrate that granulophysin is localized to the dense granules of platelets and is redistributed to the plasma membrane after platelet activation.",
author = "Israels, {S. J.} and Gerrard, {J. M.} and Jacques, {Y. V.} and A. McNicol and B. Cham and Masahiro Nishibori and Bainton, {D. F.}",
year = "1992",
language = "English",
volume = "80",
pages = "143--152",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "1",

}

TY - JOUR

T1 - Platelet dense granule membranes contain both granulophysin and P-selectin (GMP-140)

AU - Israels, S. J.

AU - Gerrard, J. M.

AU - Jacques, Y. V.

AU - McNicol, A.

AU - Cham, B.

AU - Nishibori, Masahiro

AU - Bainton, D. F.

PY - 1992

Y1 - 1992

N2 - We recently reported the characterization of a platelet granule membrane protein of molecular weight (mol wt) 40,000 called granulophysin (Gerrard et al: Blood 77:101, 1991), identified by a monoclonal antibody (MoAb D545) raised to purified dense granule membranes. Using immunoelectron-microscopic techniques on frozen thin sections, this protein was localized in resting and thrombin-stimulated platelets. In resting platelets, labeled with antigranulophysin antibodies and immunogold probes, label was localized to the membranes of one or two clear granules per platelet thin section. D545 also labeled dense granules in permeabilized whole platelets and isolated dense granule preparations examined by whole-mount techniques. Expression of granulophysin on the platelet surface paralleled dense granule secretion as measured by 14C-serotonin release under conditions in which lysosomal granule release, as measured by β-glucuronidase secretion, was less than 5%. After thrombin stimulation, both the surface-connected canalicular system and the plasma membrane were labeled, demonstrating redistribution of granulophysin associated with degranulation. Double labeling experiments with D545 and antibodies to the α-granule membrane protein, P-selectin, demonstrated labeling of both P-selectin and granulophysin on dense granule membranes. Distribution of both proteins on the plasma membrane after platelet stimulation was similar. The results demonstrate that granulophysin is localized to the dense granules of platelets and is redistributed to the plasma membrane after platelet activation.

AB - We recently reported the characterization of a platelet granule membrane protein of molecular weight (mol wt) 40,000 called granulophysin (Gerrard et al: Blood 77:101, 1991), identified by a monoclonal antibody (MoAb D545) raised to purified dense granule membranes. Using immunoelectron-microscopic techniques on frozen thin sections, this protein was localized in resting and thrombin-stimulated platelets. In resting platelets, labeled with antigranulophysin antibodies and immunogold probes, label was localized to the membranes of one or two clear granules per platelet thin section. D545 also labeled dense granules in permeabilized whole platelets and isolated dense granule preparations examined by whole-mount techniques. Expression of granulophysin on the platelet surface paralleled dense granule secretion as measured by 14C-serotonin release under conditions in which lysosomal granule release, as measured by β-glucuronidase secretion, was less than 5%. After thrombin stimulation, both the surface-connected canalicular system and the plasma membrane were labeled, demonstrating redistribution of granulophysin associated with degranulation. Double labeling experiments with D545 and antibodies to the α-granule membrane protein, P-selectin, demonstrated labeling of both P-selectin and granulophysin on dense granule membranes. Distribution of both proteins on the plasma membrane after platelet stimulation was similar. The results demonstrate that granulophysin is localized to the dense granules of platelets and is redistributed to the plasma membrane after platelet activation.

UR - http://www.scopus.com/inward/record.url?scp=0026681436&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026681436&partnerID=8YFLogxK

M3 - Article

VL - 80

SP - 143

EP - 152

JO - Blood

JF - Blood

SN - 0006-4971

IS - 1

ER -