Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis

Wei Yung Ding, Hui Ting Ong, Yusuke Hara, Jantana Wongsantichon, Yusuke Toyama, Robert C. Robinson, François Nédélec, Ronen Zaidel-Bar

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28 Citations (Scopus)

Abstract

The cell cortex is essential to maintain animal cell shape, and contractile forces generated within it by nonmuscle myosin II (NMY-2) drive cellular morphogenetic processes such as cytokinesis. The role of actin cross-linking proteins in cortical dynamics is still incompletely understood. Here, we show that the evolutionarily conserved actin bundling/cross-linking protein plastin is instrumental for the generation of potent cortical actomyosin contractility in the Caenorhabditis elegans zygote. PLST-1 was enriched in contractile structures and was required for effective coalescence of NMY-2 filaments into large contractile foci and for long-range coordinated contractility in the cortex. In the absence of PLST-1, polarization was compromised, cytokinesis was delayed or failed, and 50% of embryos died during development. Moreover, mathematical modeling showed that an optimal amount of bundling agents enhanced the ability of a network to contract. We propose that by increasing the connectivity of the F-actin meshwork, plastin enables the cortex to generate stronger and more coordinated forces to accomplish cellular morphogenesis.

Original languageEnglish
Pages (from-to)1371-1386
Number of pages16
JournalJournal of Cell Biology
Volume216
Issue number5
DOIs
Publication statusPublished - May 1 2017

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Ding, W. Y., Ong, H. T., Hara, Y., Wongsantichon, J., Toyama, Y., Robinson, R. C., Nédélec, F., & Zaidel-Bar, R. (2017). Plastin increases cortical connectivity to facilitate robust polarization and timely cytokinesis. Journal of Cell Biology, 216(5), 1371-1386. https://doi.org/10.1083/jcb.201603070