Plasma membrane localization is essential for oryza sativa pto-interacting protein 1a-mediated negative regulation of immune signaling in rice

Hidenori Matsui, Hidenori Matsui, Masayuki Fujiwara, Satoshi Hamada, K. Shimamoto, Yuko Nomura, Hirofumi Nakagami, Akira Takahashi, Hirohiko Hirochika

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Oryza sativa Pto-interacting protein 1a (OsPti1a), an ortholog of tomato (Solanum lycopersicum) SlPti1, functions as a negative regulator of innate immunity in rice (Oryza sativa). In ospti1a mutants, the activation of immune responses, including hypersensitive response-like cell death, is caused by loss of the OsPti1a protein; however, it is as yet unclear how OsPti1a suppresses immune responses. Here, we report that OsPti1a localizes to detergent-resistant membrane fractions of the plasma membrane through lipid modification of the protein’s amino terminus, which is highly conserved among Pti1 orthologs in several plant species. Importantly, mislocalization of OsPti1a after deletion of its amino terminus reduced its ability to complement the mutant phenotypes, including hypersensitive response-like cell death. Furthermore, complex formation of OsPti1a depends on its amino terminus-mediated membrane localization. Liquid chromatography-tandem mass spectrometry analysis of OsPti1a complexinteracting proteins identified several defense-related proteins. Collectively, these findings indicate that appropriate complex formation by OsPti1a at the plasma membrane is required for the negative regulation of plant immune responses in rice.

Original languageEnglish
Pages (from-to)327-336
Number of pages10
JournalPlant Physiology
Volume166
Issue number1
DOIs
Publication statusPublished - Sep 1 2014
Externally publishedYes

Fingerprint

Oryza sativa
plasma membrane
Cell Membrane
rice
Proteins
proteins
immune response
hypersensitive response
Lycopersicon esculentum
Oryza
cell death
Cell Death
Plant Immunity
mutants
Membranes
Solanum lycopersicum
Membrane Lipids
Tandem Mass Spectrometry
Innate Immunity
Liquid Chromatography

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology
  • Medicine(all)

Cite this

Plasma membrane localization is essential for oryza sativa pto-interacting protein 1a-mediated negative regulation of immune signaling in rice. / Matsui, Hidenori; Matsui, Hidenori; Fujiwara, Masayuki; Hamada, Satoshi; Shimamoto, K.; Nomura, Yuko; Nakagami, Hirofumi; Takahashi, Akira; Hirochika, Hirohiko.

In: Plant Physiology, Vol. 166, No. 1, 01.09.2014, p. 327-336.

Research output: Contribution to journalArticle

Matsui, H, Matsui, H, Fujiwara, M, Hamada, S, Shimamoto, K, Nomura, Y, Nakagami, H, Takahashi, A & Hirochika, H 2014, 'Plasma membrane localization is essential for oryza sativa pto-interacting protein 1a-mediated negative regulation of immune signaling in rice', Plant Physiology, vol. 166, no. 1, pp. 327-336. https://doi.org/10.1104/pp.114.243873
Matsui, Hidenori ; Matsui, Hidenori ; Fujiwara, Masayuki ; Hamada, Satoshi ; Shimamoto, K. ; Nomura, Yuko ; Nakagami, Hirofumi ; Takahashi, Akira ; Hirochika, Hirohiko. / Plasma membrane localization is essential for oryza sativa pto-interacting protein 1a-mediated negative regulation of immune signaling in rice. In: Plant Physiology. 2014 ; Vol. 166, No. 1. pp. 327-336.
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