Plasma HDL reduces nonesterified fatty acid hydroperoxides originating from oxidized LDL: A mechanism for its antioxidant ability

Mari Kotosai, Sachiko Shimada, Mai Kanda, Namiko Matsuda, Keiko Sekido, Yoshibumi Shimizu, Akira Tokumura, Toshiyuki Nakamura, Kaeko Murota, Yoshichika Kawai, Junji Terao

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The antioxidant property of plasma high-density lipoprotein (HDL) is thought to be involved in potential anti-atherogenic effects but the exact mechanism is not known. We aimed to reveal the contribution of HDL on the elimination of lipid hydroperoxides (LOOH) derived from oxidized low-density lipoprotein (LDL). Oxidized LDL prepared by copper ion-induced oxidation contained nonesterified fatty acid hydroperoxides (FFA-OOH) and lysophosphatidylcholine (lysoPtdCho), in addition to cholesteryl ester hydroperoxides (CE-OOH) and phosphatidylcholine hydroperoxides (PtdCho-OOH). A platelet-activating factor-acetylhydrolase (PAF-AH) inhibitor suppressed formation of FFA-OOH and lysoPtdCho in oxidized LDL. Among LOOH species, FFA-OOH was preferentially reduced by incubating oxidized LDL with HDL. HDL exhibited selective FFA-OOH reducing ability if it was mixed with a liposomal solution containing FFA-OOH, CE-OOH and PtdCho-OOH. Two-electron reduction of the hydroperoxy group to the hydroxy group was confirmed by the formation of 13-hydroxyoctadecadienoic acid from 13-hydroperoxyoctadecadienoic acid in HPLC analyses. This reducing effect was also found in apolipoprotein A-1 (apoA-1). FFA-OOH released from PtdCho-OOH due to PAF-AH activity in oxidized LDL undergo two-electron reduction by the reducing ability of apoA1 in HDL. This preferential reduction of FFA-OOH may participate in the mechanism of the antioxidant property of HDL.

Original languageEnglish
Pages (from-to)569-578
Number of pages10
JournalLipids
Volume48
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

Fingerprint

Lipid Peroxides
HDL Lipoproteins
Nonesterified Fatty Acids
Antioxidants
Plasmas
Lysophosphatidylcholines
Platelet Activating Factor
Electrons
Apolipoprotein A-I
oxidized low density lipoprotein
Copper
High Pressure Liquid Chromatography
Ions
Oxidation
Acids
cholesteryl ester hydroperoxide

Keywords

  • Apolipoprotein A-1
  • Atherosclerosis
  • HDL
  • Nonesterified fatty acid hydroperoxides
  • Oxidized LDL
  • Platelet activating factor-acetyl hydrolase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Organic Chemistry

Cite this

Plasma HDL reduces nonesterified fatty acid hydroperoxides originating from oxidized LDL : A mechanism for its antioxidant ability. / Kotosai, Mari; Shimada, Sachiko; Kanda, Mai; Matsuda, Namiko; Sekido, Keiko; Shimizu, Yoshibumi; Tokumura, Akira; Nakamura, Toshiyuki; Murota, Kaeko; Kawai, Yoshichika; Terao, Junji.

In: Lipids, Vol. 48, No. 6, 06.2013, p. 569-578.

Research output: Contribution to journalArticle

Kotosai, M, Shimada, S, Kanda, M, Matsuda, N, Sekido, K, Shimizu, Y, Tokumura, A, Nakamura, T, Murota, K, Kawai, Y & Terao, J 2013, 'Plasma HDL reduces nonesterified fatty acid hydroperoxides originating from oxidized LDL: A mechanism for its antioxidant ability', Lipids, vol. 48, no. 6, pp. 569-578. https://doi.org/10.1007/s11745-013-3779-1
Kotosai, Mari ; Shimada, Sachiko ; Kanda, Mai ; Matsuda, Namiko ; Sekido, Keiko ; Shimizu, Yoshibumi ; Tokumura, Akira ; Nakamura, Toshiyuki ; Murota, Kaeko ; Kawai, Yoshichika ; Terao, Junji. / Plasma HDL reduces nonesterified fatty acid hydroperoxides originating from oxidized LDL : A mechanism for its antioxidant ability. In: Lipids. 2013 ; Vol. 48, No. 6. pp. 569-578.
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AU - Matsuda, Namiko

AU - Sekido, Keiko

AU - Shimizu, Yoshibumi

AU - Tokumura, Akira

AU - Nakamura, Toshiyuki

AU - Murota, Kaeko

AU - Kawai, Yoshichika

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