TY - JOUR
T1 - Plant growth-promoting bacteria as potential bio-inoculants and biocontrol agents to promote black pepper plant cultivation
AU - Lau, Ee Tiing
AU - Tani, Akio
AU - Khew, Choy Yuen
AU - Chua, Yee Qin
AU - Hwang, Siaw San
N1 - Funding Information:
Sincere thanks to Group of Plant-Microbe Interactions, Biotic Stress Unit, Institute of Plant Science and Resources (IPSR), Okayama University, Japan on the technical advices and supports. This research was financially supported by Malaysian Pepper Board, Malaysia (Fund No. E016B00).
Funding Information:
Sincere thanks to Group of Plant-Microbe Interactions, Biotic Stress Unit, Institute of Plant Science and Resources (IPSR), Okayama University, Japan on the technical advices and supports. This research was financially supported by Malaysian Pepper Board , Malaysia (Fund No. E016B00 ).
Publisher Copyright:
© 2020 Elsevier GmbH
PY - 2020/11
Y1 - 2020/11
N2 - Black pepper production in Malaysia was restricted by various diseases. Hazardous chemical products appear to be the best solution to control diseases in black pepper cultivation. However, persistence of chemical residues in peppercorns could affect the quality of exports and consumptions. Application of fertilizers is crucial to sustain pepper growth and high yield. But, continuous use of chemical fertilizers could affect the soil ecosystem and eventually restrict nutrient uptake by pepper roots. Therefore, we propose biological approaches as an alternative solution instead of chemical products to sustain pepper cultivation in Malaysia. In this study, we have isolated a total of seven indigenous rhizobacteria antagonistic to soil-borne Fusarium solani, the causal fungus of slow decline, the most serious debilitating disease of black pepper in Malaysia. The isolated bacteria were identified as Bacillus subtilis, Bacillus siamensis, Brevibacillus gelatini, Pseudomonas geniculata, Pseudomonas beteli, Burkholderia ubonensis and Burkholderia territorii. These bacteria were effective in production of antifungal siderophore with the amount of 53.4 %–73.5 % per 0.5 mL of cell-free supernatants. The bacteria also produced appreciable amount of chitinase with chitinolytic index was ranged from 1.19 to 1.76. The bacteria have shown phosphate solubilizing index within 1.61 to 2.01. They were also efficient in ACC deaminase (0.52 mM–0.62 mM) and ammonia (60.3 mM–75.3 mM) production. The isolated antagonists were efficacious in stimulation of black pepper plant growth and root development through IAA (10.5 μg/mL–42.6 μg/mL) secretion. In conclusion, the isolated rhizobacteria are potent to be developed not only as biocontrol agents to minimize the utilization of hazardous chemicals in black pepper disease management, but also developed as bio-fertilizers to improve black pepper plant growth due to their capabilities in plant growth-promotion.
AB - Black pepper production in Malaysia was restricted by various diseases. Hazardous chemical products appear to be the best solution to control diseases in black pepper cultivation. However, persistence of chemical residues in peppercorns could affect the quality of exports and consumptions. Application of fertilizers is crucial to sustain pepper growth and high yield. But, continuous use of chemical fertilizers could affect the soil ecosystem and eventually restrict nutrient uptake by pepper roots. Therefore, we propose biological approaches as an alternative solution instead of chemical products to sustain pepper cultivation in Malaysia. In this study, we have isolated a total of seven indigenous rhizobacteria antagonistic to soil-borne Fusarium solani, the causal fungus of slow decline, the most serious debilitating disease of black pepper in Malaysia. The isolated bacteria were identified as Bacillus subtilis, Bacillus siamensis, Brevibacillus gelatini, Pseudomonas geniculata, Pseudomonas beteli, Burkholderia ubonensis and Burkholderia territorii. These bacteria were effective in production of antifungal siderophore with the amount of 53.4 %–73.5 % per 0.5 mL of cell-free supernatants. The bacteria also produced appreciable amount of chitinase with chitinolytic index was ranged from 1.19 to 1.76. The bacteria have shown phosphate solubilizing index within 1.61 to 2.01. They were also efficient in ACC deaminase (0.52 mM–0.62 mM) and ammonia (60.3 mM–75.3 mM) production. The isolated antagonists were efficacious in stimulation of black pepper plant growth and root development through IAA (10.5 μg/mL–42.6 μg/mL) secretion. In conclusion, the isolated rhizobacteria are potent to be developed not only as biocontrol agents to minimize the utilization of hazardous chemicals in black pepper disease management, but also developed as bio-fertilizers to improve black pepper plant growth due to their capabilities in plant growth-promotion.
KW - Bio-fertilizers
KW - Biocontrol
KW - Black pepper
KW - Diseases
KW - Rhizobacteria
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U2 - 10.1016/j.micres.2020.126549
DO - 10.1016/j.micres.2020.126549
M3 - Article
C2 - 32688172
AN - SCOPUS:85087965008
VL - 240
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
M1 - 126549
ER -