Abstract
When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.
| Original language | English |
|---|---|
| Pages (from-to) | 12-17 |
| Number of pages | 6 |
| Journal | Journal of General Plant Pathology |
| Volume | 79 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 2013 |
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Keywords
- Cell wall-associated ATPase (apyrase)
- Elicitor
- Ion efflux
- Mycosphaerella pinodes
- Pea (Pisum sativum L.)
- Suppressor
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science
Cite this
Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.). / Amano, Masashi; Toyoda, Kazuhiro; Kiba, Akinori; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.
In: Journal of General Plant Pathology, Vol. 79, No. 1, 01.2013, p. 12-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.)
AU - Amano, Masashi
AU - Toyoda, Kazuhiro
AU - Kiba, Akinori
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
PY - 2013/1
Y1 - 2013/1
N2 - When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.
AB - When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.
KW - Cell wall-associated ATPase (apyrase)
KW - Elicitor
KW - Ion efflux
KW - Mycosphaerella pinodes
KW - Pea (Pisum sativum L.)
KW - Suppressor
UR - http://www.scopus.com/inward/record.url?scp=84872173725&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84872173725&partnerID=8YFLogxK
U2 - 10.1007/s10327-012-0418-2
DO - 10.1007/s10327-012-0418-2
M3 - Article
VL - 79
SP - 12
EP - 17
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 1
ER -