Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.)

Masashi Amano, Kazuhiro Toyoda, Akinori Kiba, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.

Original languageEnglish
Pages (from-to)12-17
Number of pages6
JournalJournal of General Plant Pathology
Volume79
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

induced resistance
Vigna unguiculata
cowpeas
Pisum sativum
peas
potassium
cell walls
sodium
ions
Mycosphaerella pinodes
apyrase
epicotyls
sulfonamides
superoxide anion
biphenyl
adenosinetriphosphatase
elicitors
genes
tissues

Keywords

  • Cell wall-associated ATPase (apyrase)
  • Elicitor
  • Ion efflux
  • Mycosphaerella pinodes
  • Pea (Pisum sativum L.)
  • Suppressor

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.). / Amano, Masashi; Toyoda, Kazuhiro; Kiba, Akinori; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 79, No. 1, 01.2013, p. 12-17.

Research output: Contribution to journalArticle

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