TY - JOUR
T1 - Plant cell walls as suppliers of potassium and sodium ions for induced resistance in pea (Pisum sativum L.) and cowpea (Vigna unguiculata L.)
AU - Amano, Masashi
AU - Toyoda, Kazuhiro
AU - Kiba, Akinori
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
N1 - Funding Information:
Acknowledgments We thank Ms. K. Tanaka in our laboratory for technical assistance. This research was supported in part by the Grants-in-Aids for Scientific Research (No. 22580051) from the Japan Society for Promotion of Science (JSPS).
PY - 2013/1
Y1 - 2013/1
N2 - When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.
AB - When an elicitor is applied to plants to induce resistance, one of the first detectable events is the efflux of ions from the treated tissue. Here we are the first to demonstrate that an elicitor from Mycosphaerella pinodes evokes leakage of Na+ and K+ ions from isolated cell walls of pea and cowpea in vitro, as observed for epicotyl tissues. Pharmacological experiments showed that this elicitor-stimulated leakage was sensitive to vanadate and N-(3-methylphenyl)biphenyl-4-sulfonamide (NGXT-191), that inhibit a cell wall-associated ATPase (apyrase). Vanadate or NGXT-191 suppressed elicitor-induced superoxide generation and expression of defense genes in vivo. On the basis of these results, we assume that the leakage of these ions, probably associated with an ATP-dependent process(es) in the cell wall, is likely associated with induced defenses of pea and cowpea.
KW - Cell wall-associated ATPase (apyrase)
KW - Elicitor
KW - Ion efflux
KW - Mycosphaerella pinodes
KW - Pea (Pisum sativum L.)
KW - Suppressor
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U2 - 10.1007/s10327-012-0418-2
DO - 10.1007/s10327-012-0418-2
M3 - Article
AN - SCOPUS:84872173725
VL - 79
SP - 12
EP - 17
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 1
ER -