PK11195 might selectively suppress the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells

Sotaro Momosaki; Natsumi Imamoto; Rie Hosoi; Yoshikazu Sawada; Kohji Abe; Ming Rong Zhang; Osamu Inoue

doi:10.1016/j.brainres.2010.04.063

PK11195 might selectively suppress the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells

Sotaro Momosaki, Natsumi Imamoto, Rie Hosoi, Yoshikazu Sawada, Kohji Abe, Ming Rong Zhang, Osamu Inoue

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

PK11195 was previously reported to attenuate the quinolinic acid (QUIN)-induced enhancement of glucose metabolism in rat brain. In the present study, the effect of PK11195 or anesthesia on [¹⁴C]2-deoxyglucose ([¹⁴C]DG) uptake was investigated in order to determine whether the QUIN-induced enhancement of glucose metabolism occurred in glial cells or neurons. We confirmed that the microinjection of QUIN caused a significant enhancement of [¹⁴C]DG uptake at 2 h after the infusion, while the co-injection of PK11195 and QUIN almost completely suppressed this enhancement of [¹⁴C]DG uptake. No effect of chloral hydrate anesthesia on the QUIN-induced enhancement of [¹⁴C]DG uptake was observed. In contrast to rats treated with QUIN, PK11195 did not affect the enhancement of [¹⁴C]DG uptake induced by fluorocitrate (FC); however, chloral hydrate anesthesia completely suppressed the FC-induced increase in [¹⁴C]DG uptake. These results indicated that the enhancement of glucose metabolism induced by QUIN mainly occurred in glial cells, and the neuroprotective effect of PK11195 in rats injected with QUIN might be related to the suppression of anaerobic glycolysis in glial cells.

Original language	English
Pages (from-to)	18-23
Number of pages	6
Journal	Brain Research
Volume	1340
DOIs	https://doi.org/10.1016/j.brainres.2010.04.063
Publication status	Published - Jun 22 2010
Externally published	Yes

Keywords

Glial cell
Glucose metabolism
PK11195
Quinolinic acid
Rat brain

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/j.brainres.2010.04.063

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title = "PK11195 might selectively suppress the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells",

abstract = "PK11195 was previously reported to attenuate the quinolinic acid (QUIN)-induced enhancement of glucose metabolism in rat brain. In the present study, the effect of PK11195 or anesthesia on [14C]2-deoxyglucose ([14C]DG) uptake was investigated in order to determine whether the QUIN-induced enhancement of glucose metabolism occurred in glial cells or neurons. We confirmed that the microinjection of QUIN caused a significant enhancement of [14C]DG uptake at 2 h after the infusion, while the co-injection of PK11195 and QUIN almost completely suppressed this enhancement of [14C]DG uptake. No effect of chloral hydrate anesthesia on the QUIN-induced enhancement of [14C]DG uptake was observed. In contrast to rats treated with QUIN, PK11195 did not affect the enhancement of [14C]DG uptake induced by fluorocitrate (FC); however, chloral hydrate anesthesia completely suppressed the FC-induced increase in [14C]DG uptake. These results indicated that the enhancement of glucose metabolism induced by QUIN mainly occurred in glial cells, and the neuroprotective effect of PK11195 in rats injected with QUIN might be related to the suppression of anaerobic glycolysis in glial cells.",

keywords = "Glial cell, Glucose metabolism, PK11195, Quinolinic acid, Rat brain",

author = "Sotaro Momosaki and Natsumi Imamoto and Rie Hosoi and Yoshikazu Sawada and Kohji Abe and Zhang, {Ming Rong} and Osamu Inoue",

note = "Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research (Basic Research B: Inoue O., No. 19390319 ) from the Ministry of Education, Culture, Sports, Science & Technology of the Japanese Government .",

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T1 - PK11195 might selectively suppress the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells

AU - Momosaki, Sotaro

AU - Imamoto, Natsumi

AU - Hosoi, Rie

AU - Sawada, Yoshikazu

AU - Abe, Kohji

AU - Zhang, Ming Rong

AU - Inoue, Osamu

N1 - Funding Information: This study was supported in part by Grants-in-Aid for Scientific Research (Basic Research B: Inoue O., No. 19390319 ) from the Ministry of Education, Culture, Sports, Science & Technology of the Japanese Government .

PY - 2010/6/22

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AB - PK11195 was previously reported to attenuate the quinolinic acid (QUIN)-induced enhancement of glucose metabolism in rat brain. In the present study, the effect of PK11195 or anesthesia on [14C]2-deoxyglucose ([14C]DG) uptake was investigated in order to determine whether the QUIN-induced enhancement of glucose metabolism occurred in glial cells or neurons. We confirmed that the microinjection of QUIN caused a significant enhancement of [14C]DG uptake at 2 h after the infusion, while the co-injection of PK11195 and QUIN almost completely suppressed this enhancement of [14C]DG uptake. No effect of chloral hydrate anesthesia on the QUIN-induced enhancement of [14C]DG uptake was observed. In contrast to rats treated with QUIN, PK11195 did not affect the enhancement of [14C]DG uptake induced by fluorocitrate (FC); however, chloral hydrate anesthesia completely suppressed the FC-induced increase in [14C]DG uptake. These results indicated that the enhancement of glucose metabolism induced by QUIN mainly occurred in glial cells, and the neuroprotective effect of PK11195 in rats injected with QUIN might be related to the suppression of anaerobic glycolysis in glial cells.

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KW - Glucose metabolism

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KW - Quinolinic acid

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PK11195 might selectively suppress the quinolinic acid-induced enhancement of anaerobic glycolysis in glial cells

Abstract

Keywords

ASJC Scopus subject areas

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