Abstract
Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.
| Original language | English |
|---|---|
| Journal | Journal of Clinical Pathology |
| DOIs | |
| Publication status | Accepted/In press - Jan 1 2019 |
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Keywords
- cancer
- lung
- staining
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Pilot evaluation of a HER2 testing in non-small-cell lung cancer. / Hotta, Katsuyuki; Yanai, Hiroyuki; Ohashi, Kadoaki; Ninomiya, Kiichiro; Nakashima, Hiromi; Kayatani, Hiroe; Takata, Minoru; Kiura, Katsuyuki.
In: Journal of Clinical Pathology, 01.01.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pilot evaluation of a HER2 testing in non-small-cell lung cancer
AU - Hotta, Katsuyuki
AU - Yanai, Hiroyuki
AU - Ohashi, Kadoaki
AU - Ninomiya, Kiichiro
AU - Nakashima, Hiromi
AU - Kayatani, Hiroe
AU - Takata, Minoru
AU - Kiura, Katsuyuki
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.
AB - Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.
KW - cancer
KW - lung
KW - staining
UR - http://www.scopus.com/inward/record.url?scp=85075989817&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85075989817&partnerID=8YFLogxK
U2 - 10.1136/jclinpath-2019-206204
DO - 10.1136/jclinpath-2019-206204
M3 - Article
C2 - 31796633
AN - SCOPUS:85075989817
JO - Journal of Clinical Pathology - Clinical Molecular Pathology
JF - Journal of Clinical Pathology - Clinical Molecular Pathology
SN - 0021-9746
ER -