Pilot evaluation of a HER2 testing in non-small-cell lung cancer

Katsuyuki Hotta; Hiroyuki Yanai; Kadoaki Ohashi; Kiichiro Ninomiya; Hiromi Nakashima; Hiroe Kayatani; Minoru Takata; Katsuyuki Kiura

doi:10.1136/jclinpath-2019-206204

Pilot evaluation of a HER2 testing in non-small-cell lung cancer

Katsuyuki Hotta, Hiroyuki Yanai, Kadoaki Ohashi, Kiichiro Ninomiya, Hiromi Nakashima, Hiroe Kayatani, Minoru Takata, Katsuyuki Kiura

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1 Citation (Scopus)

Abstract

Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.

Original language	English
Journal	Journal of Clinical Pathology
DOIs	https://doi.org/10.1136/jclinpath-2019-206204
Publication status	Accepted/In press - Jan 1 2019

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Keywords

cancer
lung
staining

ASJC Scopus subject areas

Pathology and Forensic Medicine

Cite this

Hotta, K., Yanai, H., Ohashi, K., Ninomiya, K., Nakashima, H., Kayatani, H., Takata, M., & Kiura, K. (Accepted/In press). Pilot evaluation of a HER2 testing in non-small-cell lung cancer. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2019-206204

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abstract = "Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.",

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author = "Katsuyuki Hotta and Hiroyuki Yanai and Kadoaki Ohashi and Kiichiro Ninomiya and Hiromi Nakashima and Hiroe Kayatani and Minoru Takata and Katsuyuki Kiura",

year = "2019",

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AU - Hotta, Katsuyuki

AU - Yanai, Hiroyuki

AU - Ohashi, Kadoaki

AU - Ninomiya, Kiichiro

AU - Nakashima, Hiromi

AU - Kayatani, Hiroe

AU - Takata, Minoru

AU - Kiura, Katsuyuki

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.

AB - Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.

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10.1136/jclinpath-2019-206204