Pilot evaluation of a HER2 testing in non-small-cell lung cancer

Katsuyuki Hotta, Hiroyuki Yanai, Kadoaki Ohashi, Kiichiro Ninomiya, Hiromi Nakashima, Hiroe Kayatani, Minoru Takata, Katsuyuki Kiura

Research output: Contribution to journalArticle

Abstract

Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.

Original languageEnglish
JournalJournal of Clinical Pathology
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Non-Small Cell Lung Carcinoma
Immunohistochemistry
Breast Neoplasms
Stomach Neoplasms
Formaldehyde
In Situ Hybridization
Cohort Studies
Prospective Studies
Neoplasms

Keywords

  • cancer
  • lung
  • staining

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Pilot evaluation of a HER2 testing in non-small-cell lung cancer. / Hotta, Katsuyuki; Yanai, Hiroyuki; Ohashi, Kadoaki; Ninomiya, Kiichiro; Nakashima, Hiromi; Kayatani, Hiroe; Takata, Minoru; Kiura, Katsuyuki.

In: Journal of Clinical Pathology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79{\%}) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5{\%} according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8{\%}) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8{\%}. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.",
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AU - Ohashi, Kadoaki

AU - Ninomiya, Kiichiro

AU - Nakashima, Hiromi

AU - Kayatani, Hiroe

AU - Takata, Minoru

AU - Kiura, Katsuyuki

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N2 - Aims: HER2-positivity pattern in the specimens of immunohistochemistry (IHC) and ? uorescence in situ hybridisation (FISH) has been hardly reported in non-small-cell lung cancer (NSCLC). Methods: We evaluated the characteristics of HER2-positivity pattern in formalin-? xed paraf? n-embedded samples using IHC and FISH in 15 patients enrolled in a larger prospective cohort study to survey a HER2-positive NSCLC. Results: As for the immunostaining pattern, most specimens (79%) demonstrated incomplete or mixed-typed membranous immunoreactivity with heterogeneity, resembling that observed in gastric cancer rather than breast cancer. Concordance between IHC-positivity and FISH-positivity was 87.5% according to the criteria for breast cancer scoring system. On application of the gastric cancer scoring system to the examined tumours, the IHC score increased in the seven (43.8%) specimens, and the concordance between IHC positivity and FISH positivity rose to 93.8%. Conclusions: In our pilot series, the pattern of IHC reactivity closely resembled that observed in gastric cancer rather than breast cancer.

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