piggyBac-mediated somatic transformation of the two-spotted cricket, Gryllus bimaculatus

Yohei Shinmyo, Taro Mito, Takashi Matsushita, Isao Sarashina, Katsuyuki Miyawaki, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Transgenic insects have been artificially produced to study functions of interesting developmental genes, using insect transposons such as piggyBac. In the case of the cricket, however, transgenic animals have not yet been successfully artificially produced. In the present study, we examined whether the piggyBac transposon functions as a tool for gene delivery in embryos of Gryllus bimaculatus. We used either a piggyBac helper plasmid or a helper RNA synthesized in vitro as a transposase source. An excision assay revealed that the helper RNA was more effective in early Gryllus eggs to transpose a marker gene of eGFP than the helper plasmid containing the piggyBac transposase gene driven by the G. bimaculatus actin3/4 promoter. Further, only when the helper RNA was used, somatic transformation of the embryo with the eGFP gene was observed. These results suggest that the piggyBac system with the helper RNA may be effective for making transgenic crickets.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalDevelopment Growth and Differentiation
Volume46
Issue number4
DOIs
Publication statusPublished - Aug 1 2004
Externally publishedYes

    Fingerprint

Keywords

  • Gryllus bimaculatus
  • Helper RNA
  • Transgenic cricket
  • eGFP
  • piggyBac

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this