Piccolo, a novel 420 kDa protein associated with the presynaptic cytomatrix

Claudia Cases-Langhoff, Britta Voss, Abigail M. Garner, Ute Appeltauer, Koji Takei, Stefan Kindler, Rüdiger W. Veh, Pietro De Camilli, Eckart D. Gundelfinger, Craig C. Garner

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129 Citations (Scopus)

Abstract

In this study, we describe a novel 420 kDa protein, called Piccolo, found at a wide variety of adult rat brain synapses. High protein levels in the cerebellum, the olfactory bulb and the hippocampus were frequently observed to be associated with asymmetric type 1 synapses. Piccolo is selectively enriched in presynaptic terminals, but is not a component of synaptic vesicles (SVs). Immunogold electron microscopy revealed that Piccolo localizes to the amorphous material among SVs at the presynaptic plasma membrane. Biochemical studies showed that it is very tightly bound to this structure. Thus, we speculate that Piccolo is a structural component of the presynaptic cytomatrix which anchors SVs to the presynaptic plasmalemma.

Original languageEnglish
Pages (from-to)214-223
Number of pages10
JournalEuropean Journal of Cell Biology
Volume69
Issue number3
Publication statusPublished - Mar 1996
Externally publishedYes

Keywords

  • Cytoskeleton
  • Electron microscopy
  • Neurons
  • Rat brain
  • Synaptic junction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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  • Cite this

    Cases-Langhoff, C., Voss, B., Garner, A. M., Appeltauer, U., Takei, K., Kindler, S., Veh, R. W., De Camilli, P., Gundelfinger, E. D., & Garner, C. C. (1996). Piccolo, a novel 420 kDa protein associated with the presynaptic cytomatrix. European Journal of Cell Biology, 69(3), 214-223.