Phytoseiid mite species composition in Japanese peach orchards estimated using quantitative sequencing

Shoji Sonoda, Yoko Kohara, Siqingerile, Shingo Toyoshima, Hidenari Kishimoto, Norihide Hinomoto

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)

    Abstract

    We attempted a population survey of spider mites and phytoseiid mites in Japanese peach orchards with different pesticide practices; however, we had difficulty discriminating phytoseiid mites. To estimate phytoseiid mite species composition, ribosomal gene fragments were amplified from genomic DNA of five phytoseiid mite species using PCR. Cloning and nucleotide sequencing of amplified fragments identified species-specific polymorphic sites. Newly amplified fragments from recombinant plasmids were mixed in various ratios to produce standard DNA template mixtures. After direct sequencing, the signal ratios between two nucleotides at each species-specific polymorphic site were calculated and shown against the corresponding expected ratios. Quadratic regression equations were used to estimate the phytoseiid mite species composition. Results showed that the phytoseiid mite species composition changed during the survey period and varied among study sites.

    Original languageEnglish
    Pages (from-to)9-22
    Number of pages14
    JournalExperimental and Applied Acarology
    Volume56
    Issue number1
    DOIs
    Publication statusPublished - Jan 2012

    Keywords

    • Peach orchard
    • Phytoseiid mite
    • Quantitative sequencing
    • Species composition
    • Spider mite

    ASJC Scopus subject areas

    • Ecology
    • Insect Science

    Fingerprint

    Dive into the research topics of 'Phytoseiid mite species composition in Japanese peach orchards estimated using quantitative sequencing'. Together they form a unique fingerprint.

    Cite this