Phytoseiid mite species composition in Japanese peach orchards estimated using quantitative sequencing

Shoji Sonoda, Yoko Kohara, Siqingerile, Shingo Toyoshima, Hidenari Kishimoto, Norihide Hinomoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We attempted a population survey of spider mites and phytoseiid mites in Japanese peach orchards with different pesticide practices; however, we had difficulty discriminating phytoseiid mites. To estimate phytoseiid mite species composition, ribosomal gene fragments were amplified from genomic DNA of five phytoseiid mite species using PCR. Cloning and nucleotide sequencing of amplified fragments identified species-specific polymorphic sites. Newly amplified fragments from recombinant plasmids were mixed in various ratios to produce standard DNA template mixtures. After direct sequencing, the signal ratios between two nucleotides at each species-specific polymorphic site were calculated and shown against the corresponding expected ratios. Quadratic regression equations were used to estimate the phytoseiid mite species composition. Results showed that the phytoseiid mite species composition changed during the survey period and varied among study sites.

Original languageEnglish
Pages (from-to)9-22
Number of pages14
JournalExperimental and Applied Acarology
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

Keywords

  • Peach orchard
  • Phytoseiid mite
  • Quantitative sequencing
  • Species composition
  • Spider mite

ASJC Scopus subject areas

  • Ecology
  • Insect Science

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