Phytopathogenic fungus hosts a plant virus

A naturally occurring cross-kingdom viral infection

Andika Ida Bagus, Shuang Wei, Chunmei Cao, Lakha Salaipeth, Hideki Kondo, Liying Sun

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The transmission of viral infections between plant and fungal hosts has been suspected to occur, based on phylogenetic and other findings, but has not been directly observed in nature. Here, we report the discovery of a natural infection of the phytopathogenic fungus Rhizoctonia solani by a plant virus, cucumber mosaic virus (CMV). The CMV-infected R. solani strain was obtained from a potato plant growing in Inner Mongolia Province of China, and CMV infection was stable when this fungal strain was cultured in the laboratory. CMV was horizontally transmitted through hyphal anastomosis but not vertically through basidiospores. By inoculation via protoplast transfection with virions, a reference isolate of CMV replicated in R. solani and another phytopathogenic fungus, suggesting that some fungi can serve as alternative hosts to CMV. Importantly, in fungal inoculation experiments under laboratory conditions, R. solani could acquire CMV from an infected plant, as well as transmit the virus to an uninfected plant. This study presents evidence of the transfer of a virus between plant and fungus, and it further expands our understanding of plant–fungus interactions and the spread of plant viruses.

Original languageEnglish
Pages (from-to)12267-12272
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number46
DOIs
Publication statusPublished - Nov 14 2017

Fingerprint

Cucumovirus
Plant Viruses
Virus Diseases
Fungi
China
Rhizoctonia
Mycoses
Protoplasts
Solanum tuberosum
Virion
Transfection
Viruses

Keywords

  • Cross-kingdom
  • Fungus
  • Plant virus
  • Transmission

ASJC Scopus subject areas

  • General

Cite this

Phytopathogenic fungus hosts a plant virus : A naturally occurring cross-kingdom viral infection. / Ida Bagus, Andika; Wei, Shuang; Cao, Chunmei; Salaipeth, Lakha; Kondo, Hideki; Sun, Liying.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 46, 14.11.2017, p. 12267-12272.

Research output: Contribution to journalArticle

@article{23df90a3d758495fa4906d735acd2d18,
title = "Phytopathogenic fungus hosts a plant virus: A naturally occurring cross-kingdom viral infection",
abstract = "The transmission of viral infections between plant and fungal hosts has been suspected to occur, based on phylogenetic and other findings, but has not been directly observed in nature. Here, we report the discovery of a natural infection of the phytopathogenic fungus Rhizoctonia solani by a plant virus, cucumber mosaic virus (CMV). The CMV-infected R. solani strain was obtained from a potato plant growing in Inner Mongolia Province of China, and CMV infection was stable when this fungal strain was cultured in the laboratory. CMV was horizontally transmitted through hyphal anastomosis but not vertically through basidiospores. By inoculation via protoplast transfection with virions, a reference isolate of CMV replicated in R. solani and another phytopathogenic fungus, suggesting that some fungi can serve as alternative hosts to CMV. Importantly, in fungal inoculation experiments under laboratory conditions, R. solani could acquire CMV from an infected plant, as well as transmit the virus to an uninfected plant. This study presents evidence of the transfer of a virus between plant and fungus, and it further expands our understanding of plant–fungus interactions and the spread of plant viruses.",
keywords = "Cross-kingdom, Fungus, Plant virus, Transmission",
author = "{Ida Bagus}, Andika and Shuang Wei and Chunmei Cao and Lakha Salaipeth and Hideki Kondo and Liying Sun",
year = "2017",
month = "11",
day = "14",
doi = "10.1073/pnas.1714916114",
language = "English",
volume = "114",
pages = "12267--12272",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "46",

}

TY - JOUR

T1 - Phytopathogenic fungus hosts a plant virus

T2 - A naturally occurring cross-kingdom viral infection

AU - Ida Bagus, Andika

AU - Wei, Shuang

AU - Cao, Chunmei

AU - Salaipeth, Lakha

AU - Kondo, Hideki

AU - Sun, Liying

PY - 2017/11/14

Y1 - 2017/11/14

N2 - The transmission of viral infections between plant and fungal hosts has been suspected to occur, based on phylogenetic and other findings, but has not been directly observed in nature. Here, we report the discovery of a natural infection of the phytopathogenic fungus Rhizoctonia solani by a plant virus, cucumber mosaic virus (CMV). The CMV-infected R. solani strain was obtained from a potato plant growing in Inner Mongolia Province of China, and CMV infection was stable when this fungal strain was cultured in the laboratory. CMV was horizontally transmitted through hyphal anastomosis but not vertically through basidiospores. By inoculation via protoplast transfection with virions, a reference isolate of CMV replicated in R. solani and another phytopathogenic fungus, suggesting that some fungi can serve as alternative hosts to CMV. Importantly, in fungal inoculation experiments under laboratory conditions, R. solani could acquire CMV from an infected plant, as well as transmit the virus to an uninfected plant. This study presents evidence of the transfer of a virus between plant and fungus, and it further expands our understanding of plant–fungus interactions and the spread of plant viruses.

AB - The transmission of viral infections between plant and fungal hosts has been suspected to occur, based on phylogenetic and other findings, but has not been directly observed in nature. Here, we report the discovery of a natural infection of the phytopathogenic fungus Rhizoctonia solani by a plant virus, cucumber mosaic virus (CMV). The CMV-infected R. solani strain was obtained from a potato plant growing in Inner Mongolia Province of China, and CMV infection was stable when this fungal strain was cultured in the laboratory. CMV was horizontally transmitted through hyphal anastomosis but not vertically through basidiospores. By inoculation via protoplast transfection with virions, a reference isolate of CMV replicated in R. solani and another phytopathogenic fungus, suggesting that some fungi can serve as alternative hosts to CMV. Importantly, in fungal inoculation experiments under laboratory conditions, R. solani could acquire CMV from an infected plant, as well as transmit the virus to an uninfected plant. This study presents evidence of the transfer of a virus between plant and fungus, and it further expands our understanding of plant–fungus interactions and the spread of plant viruses.

KW - Cross-kingdom

KW - Fungus

KW - Plant virus

KW - Transmission

UR - http://www.scopus.com/inward/record.url?scp=85033732645&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85033732645&partnerID=8YFLogxK

U2 - 10.1073/pnas.1714916114

DO - 10.1073/pnas.1714916114

M3 - Article

VL - 114

SP - 12267

EP - 12272

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 46

ER -