Phytochrome C is a key factor controlling long-day flowering in barley

Hidetaka Nishida, Daisuke Ishihara, Makoto Ishii, Takuma Kaneko, Hiroyuki Kawahigashi, Yukari Akashi, Daisuke Saisho, Katsunori Tanaka, Hirokazu Handa, Kazuyoshi Takeda, Kenji Kato

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Abstract

The spring-type near isogenic line (NIL) of the winter-type barley (Hordeum vulgare ssp. vulgare) var. Hayakiso 2 (HK2) was developed by introducing VERNALIZATION-H1 (Vrn-H1) for spring growth habit from the spring-type var. Indo Omugi. Contrary to expectations, the spring-type NIL flowered later than winter-type HK2. This phenotypic difference was controlled by a single gene, which cosegregated only with phytochrome C (HvPhyC) among three candidates around the Vrn-H1 region (Vrn-H1, HvPhyC, and CASEIN KINASE IIα), indicating that HvPhyC was the most likely candidate gene. Compared with the late-flowering allele HvPhyC-l from the NIL, the early-flowering allele HvPhyC-e from HK2 had a single nucleotide polymorphism T1139C in exon 1, which caused a nonsynonymous amino acid substitution of phenylalanine at position 380 by serine in the functionally essential GAF (39, 59-cyclic-GMP phosphodiesterase, adenylate cyclase, formate hydrogen lyase activator protein) domain. Functional assay using a rice (Oryza sativa) phyA phyC double mutant line showed that both of the HvPhyC alleles are functional, but HvPhyC-e may have a hyperfunction. Expression analysis using NILs carrying HvPhyC-e and HvPhyC-l (NIL [HvPhyC-e] and NIL [HvPhyC-l], respectively) showed that HvPhyC-e up-regulated only the flowering promoter FLOWERING LOCUS T1 by bypassing the circadian clock genes and flowering integrator CONSTANS1 under a long photoperiod. Consistent with the up-regulation, NIL (HvPhyC-e) flowered earlier than NIL (HvPhyC-l) under long photoperiods. These results implied that HvPhyC is a key factor to control long-day flowering directly.

Original languageEnglish
Pages (from-to)804-814
Number of pages11
JournalPlant physiology
Volume163
Issue number2
DOIs
Publication statusPublished - Oct 1 2013

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ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Nishida, H., Ishihara, D., Ishii, M., Kaneko, T., Kawahigashi, H., Akashi, Y., Saisho, D., Tanaka, K., Handa, H., Takeda, K., & Kato, K. (2013). Phytochrome C is a key factor controlling long-day flowering in barley. Plant physiology, 163(2), 804-814. https://doi.org/10.1104/pp.113.222570