Phyto- and endogenous estrogens differently activate intracellular calcium ion mobilization in bovine endometrial cells

Izabela Woclawek-Potocka, Krzysztof Borkowski, Anna Korzekwa, Kiyoshi Okuda, Dariusz J. Skarzynski

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The main purpose of this study was to check whether phyto- and endogenous estrogens influence calcium ion mobilization [Ca2+]i in bovine endometrial cells and whether this action is connected with biological effects i.e. prostaglandin (PG)F production. In our study we used two calcium measurement methods by comparing the microscopic method with widely used quantitative - spectrofluorometric method of [Ca2+] i measurement. We also wanted to confirm whether visualization of calcium ion [Ca2+]i in cells using microscopic method supported by micro image analysis (Micro Image Olympus system) reflects real, qualitative changes in the ion concentration. In both methods a cell-permeable form of fluorescent [Ca2+]i indicator Fura-2 was used. Cultured bovine endometrial epithelial and stromal cells influenced by phorbol-2-myristate-13-acetate (PMA; positive control), estradiol 17-β (E2; endogenous estrogen) and active metabolites of phytoestrogens (environmental estrogens) were used as a model to study PGF secretion and [Ca2+]i mobilization in the cells. Equol and para-ethyl-phenol in doses of 10-8-10-6 M increased PGF concentration both in epithelial and stromal cells (P2+] i mobilization in epithelial and stromal cells (P>0.05). Both methods revealed that only E2 and PMA induced intracellular [Ca 2+]i mobilization in epithelial and stromal cells (P2, did not cause intracellular [Ca 2+]i mobilization in endometrial cells. The obtained results proved that the [Ca2+]i visualization method supported by micro image analysis can produce similar results to the spectrofluorometric method.

Original languageEnglish
Pages (from-to)731-740
Number of pages10
JournalJournal of Reproduction and Development
Volume52
Issue number6
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Phytoestrogens
estrogens
Ions
ions
Calcium
calcium
cattle
Stromal Cells
stromal cells
Dinoprost
Epithelial Cells
epithelial cells
cells
Estrogens
methodology
Equol
image analysis
Myristic Acid
plant estrogens
Phenol

Keywords

  • Calcium measurement
  • Cattle
  • Endometrial cells
  • PGF
  • Phytoestrogens

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine

Cite this

Phyto- and endogenous estrogens differently activate intracellular calcium ion mobilization in bovine endometrial cells. / Woclawek-Potocka, Izabela; Borkowski, Krzysztof; Korzekwa, Anna; Okuda, Kiyoshi; Skarzynski, Dariusz J.

In: Journal of Reproduction and Development, Vol. 52, No. 6, 12.2006, p. 731-740.

Research output: Contribution to journalArticle

Woclawek-Potocka, Izabela ; Borkowski, Krzysztof ; Korzekwa, Anna ; Okuda, Kiyoshi ; Skarzynski, Dariusz J. / Phyto- and endogenous estrogens differently activate intracellular calcium ion mobilization in bovine endometrial cells. In: Journal of Reproduction and Development. 2006 ; Vol. 52, No. 6. pp. 731-740.
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