Physiological roles of three Na+/H+ antiporters in the halophilic bacterium Vibrio parahaemolyticus

Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Vibrio parahaemolyticus mutants lacking three Na+/H+ antiporters (NhaA, NhaB, NhaD) were constructed. The ΔnhaA strains showed significantly higher sensitivity to LiCl regarding their growth compared to the parental strain. The ΔnhaA and ΔnhaB strains exhibited higher sensitivities to LiCl. The mutant XACabd lacking all of the three antiporters could not grow in the presence of 500 mM LiCl at pH 7.0, or 50 mM at pH 8.5. The XACabd mutant was also sensitive to 1.0 M NaCl at pH 8.5. These results suggest that Na+/H+ antiporters, especially NhaA, are responsible for resistance to LiCl and to high concentrations of NaCl. Reduced Na +/H+ and Li+/H+ antiport activities were observed with everted membrane vesicles of ΔnhaB strains. However, Li+/H+ antiport activities of ΔnhaB strains were two times higher than those of ΔnhaA strains when cells were cultured at pH 8.5. It seems that expression of nhaA and nhaB is dependent on medium pH to some extent. In addition, HQNO (2-heptyl-4-hydroxyquinoline N-oxide), which is a potent inhibitor of the respiratory Na+ pump, inhibited growth of XACabd, but not of the wild type strain. Moreover, survival rate of XACabd under hypoosmotic stress was lower than that of wild type strain. It is likely that the Na+/H+ antiporters are involved in osmoregulation under hypoosmotic stress. Based on these findings, we propose that the Na +/H+ antiporters cooperate with the respiratory Na + pump in ionic homeostasis in V. parahaemolyticus.

Original languageEnglish
Pages (from-to)711-719
Number of pages9
JournalMicrobiology and Immunology
Volume49
Issue number8
Publication statusPublished - 2005

Fingerprint

Vibrio parahaemolyticus
Sodium-Hydrogen Antiporter
Bacteria
Ion Transport
Antiporters
Osmoregulation
Growth
Cultured Cells
Homeostasis
Membranes

Keywords

  • Na/H antiporter
  • NhaA
  • NhaB
  • NhaD
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Microbiology

Cite this

Physiological roles of three Na+/H+ antiporters in the halophilic bacterium Vibrio parahaemolyticus. / Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Microbiology and Immunology, Vol. 49, No. 8, 2005, p. 711-719.

Research output: Contribution to journalArticle

Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Physiological roles of three Na+/H+ antiporters in the halophilic bacterium Vibrio parahaemolyticus. In: Microbiology and Immunology. 2005 ; Vol. 49, No. 8. pp. 711-719.
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AB - Vibrio parahaemolyticus mutants lacking three Na+/H+ antiporters (NhaA, NhaB, NhaD) were constructed. The ΔnhaA strains showed significantly higher sensitivity to LiCl regarding their growth compared to the parental strain. The ΔnhaA and ΔnhaB strains exhibited higher sensitivities to LiCl. The mutant XACabd lacking all of the three antiporters could not grow in the presence of 500 mM LiCl at pH 7.0, or 50 mM at pH 8.5. The XACabd mutant was also sensitive to 1.0 M NaCl at pH 8.5. These results suggest that Na+/H+ antiporters, especially NhaA, are responsible for resistance to LiCl and to high concentrations of NaCl. Reduced Na +/H+ and Li+/H+ antiport activities were observed with everted membrane vesicles of ΔnhaB strains. However, Li+/H+ antiport activities of ΔnhaB strains were two times higher than those of ΔnhaA strains when cells were cultured at pH 8.5. It seems that expression of nhaA and nhaB is dependent on medium pH to some extent. In addition, HQNO (2-heptyl-4-hydroxyquinoline N-oxide), which is a potent inhibitor of the respiratory Na+ pump, inhibited growth of XACabd, but not of the wild type strain. Moreover, survival rate of XACabd under hypoosmotic stress was lower than that of wild type strain. It is likely that the Na+/H+ antiporters are involved in osmoregulation under hypoosmotic stress. Based on these findings, we propose that the Na +/H+ antiporters cooperate with the respiratory Na + pump in ionic homeostasis in V. parahaemolyticus.

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