Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan

Daisei Ueno, Emi Koyama, Naoki Yamaji, Jian Feng Ma

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F2 population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

Original languageEnglish
Pages (from-to)2265-2272
Number of pages8
JournalJournal of Experimental Botany
Volume62
Issue number7
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Cadmium
Molecular Biology
cadmium
rice
cultivars
Oryza
shoots
Phenotype
uptake mechanisms
Recessive Genes
phenotype
recessive genes
tonoplast
staples
Vacuoles
polluted soils
vacuoles
genetic techniques and protocols
transporters

Keywords

  • Cadmium accumulation
  • OsHMA3
  • rice
  • sequestration
  • transport
  • vacuole

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan. / Ueno, Daisei; Koyama, Emi; Yamaji, Naoki; Ma, Jian Feng.

In: Journal of Experimental Botany, Vol. 62, No. 7, 04.2011, p. 2265-2272.

Research output: Contribution to journalArticle

@article{adb1b0c172144e58973faac3602c2228,
title = "Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan",
abstract = "Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49{\%} of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F2 population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.",
keywords = "Cadmium accumulation, OsHMA3, rice, sequestration, transport, vacuole",
author = "Daisei Ueno and Emi Koyama and Naoki Yamaji and Ma, {Jian Feng}",
year = "2011",
month = "4",
doi = "10.1093/jxb/erq383",
language = "English",
volume = "62",
pages = "2265--2272",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "7",

}

TY - JOUR

T1 - Physiological, genetic, and molecular characterization of a high-Cd-accumulating rice cultivar, Jarjan

AU - Ueno, Daisei

AU - Koyama, Emi

AU - Yamaji, Naoki

AU - Ma, Jian Feng

PY - 2011/4

Y1 - 2011/4

N2 - Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F2 population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

AB - Cadmium (Cd) in rice is a major source of Cd intake for people on a staple rice diet. The mechanisms underlying Cd accumulation in rice plant are still poorly understood. Here, we characterized the physiology and genetics of Cd transport in a high-Cd-accumulating cultivar (Jarjan) of rice (Oryza sativa). Jarjan showed 5- to 34-fold higher Cd accumulation in the shoots and grains than the cultivar Nipponbare, when it was grown in either a non-Cd-contaminated or a Cd-contaminated soil. A short-term uptake experiment showed no significant difference in Cd uptake by the roots between the two cultivars. However, Jarjan translocated 49% of the total Cd taken up to the shoots, whereas Nipponbare retained most of the Cd in the roots. In both concentration- and time-dependent experiments, Jarjan showed a superior capacity for root-to-shoot translocation of Cd. These results indicate that the high-Cd-accumulation phenotype in Jarjan results from efficient translocation of Cd from roots to shoots. Genetic analysis using an F2 population derived from Jarjan and Nipponbare revealed that plants showing high- and low-Cd-accumulation phenotypes segregated in a 1:3 ratio, indicating that high accumulation in Jarjan is controlled by a single recessive gene. Furthermore, we isolated OsHMA3, a gene encoding a tonoplast-localized Cd transporter from Jarjan. The OsHMA3 protein was localized in all roots cells, but the sequence has a mutation leading to loss of function. Therefore, failure to sequester Cd into the root vacuoles by OsHMA3 is probably responsible for high Cd accumulation in Jarjan.

KW - Cadmium accumulation

KW - OsHMA3

KW - rice

KW - sequestration

KW - transport

KW - vacuole

UR - http://www.scopus.com/inward/record.url?scp=79955418106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955418106&partnerID=8YFLogxK

U2 - 10.1093/jxb/erq383

DO - 10.1093/jxb/erq383

M3 - Article

C2 - 21127026

AN - SCOPUS:79955418106

VL - 62

SP - 2265

EP - 2272

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 7

ER -