Abstract
Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.
| Original language | English |
|---|---|
| Pages (from-to) | 1837-1844 |
| Number of pages | 8 |
| Journal | Journal of Inorganic Biochemistry |
| Volume | 99 |
| Issue number | 9 SPEC. ISS. |
| DOIs | |
| Publication status | Published - Sep 2005 |
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Keywords
- Aluminium toxicity
- Aluminium-sensitive rice mutant
- Germination
- Rice
- Root growth
- Wheat
ASJC Scopus subject areas
- Biochemistry
- Inorganic Chemistry
Cite this
Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination. / Kikui, Satoshi; Sasaki, Takayuki; Maekawa, Masahiko; Miyao, Akio; Hirochika, Hirohiko; Matsumoto, Hideaki; Yamamoto, Yoko.
In: Journal of Inorganic Biochemistry, Vol. 99, No. 9 SPEC. ISS., 09.2005, p. 1837-1844.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination
AU - Kikui, Satoshi
AU - Sasaki, Takayuki
AU - Maekawa, Masahiko
AU - Miyao, Akio
AU - Hirochika, Hirohiko
AU - Matsumoto, Hideaki
AU - Yamamoto, Yoko
PY - 2005/9
Y1 - 2005/9
N2 - Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.
AB - Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.
KW - Aluminium toxicity
KW - Aluminium-sensitive rice mutant
KW - Germination
KW - Rice
KW - Root growth
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=24344456474&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24344456474&partnerID=8YFLogxK
U2 - 10.1016/j.jinorgbio.2005.06.031
DO - 10.1016/j.jinorgbio.2005.06.031
M3 - Article
VL - 99
SP - 1837
EP - 1844
JO - Journal of Inorganic Biochemistry
JF - Journal of Inorganic Biochemistry
SN - 0162-0134
IS - 9 SPEC. ISS.
ER -