Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination

Satoshi Kikui, Takayuki Sasaki, Masahiko Maekawa, Akio Miyao, Hirohiko Hirochika, Hideaki Matsumoto, Yoko Yamamoto

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.

Original languageEnglish
Pages (from-to)1837-1844
Number of pages8
JournalJournal of Inorganic Biochemistry
Volume99
Issue number9 SPEC. ISS.
DOIs
Publication statusPublished - Sep 2005

Fingerprint

Germination
Aluminum
Growth
Triticum
Seedlings
Oryza
Plant Roots
Cell membranes
Crops
Screening
Hypersensitivity
Soil
Cell Membrane
Ions

Keywords

  • Aluminium toxicity
  • Aluminium-sensitive rice mutant
  • Germination
  • Rice
  • Root growth
  • Wheat

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination. / Kikui, Satoshi; Sasaki, Takayuki; Maekawa, Masahiko; Miyao, Akio; Hirochika, Hirohiko; Matsumoto, Hideaki; Yamamoto, Yoko.

In: Journal of Inorganic Biochemistry, Vol. 99, No. 9 SPEC. ISS., 09.2005, p. 1837-1844.

Research output: Contribution to journalArticle

Kikui, Satoshi ; Sasaki, Takayuki ; Maekawa, Masahiko ; Miyao, Akio ; Hirochika, Hirohiko ; Matsumoto, Hideaki ; Yamamoto, Yoko. / Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination. In: Journal of Inorganic Biochemistry. 2005 ; Vol. 99, No. 9 SPEC. ISS. pp. 1837-1844.
@article{51676c12bb8d4384a7ffb465d68dcd1e,
title = "Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination",
abstract = "Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.",
keywords = "Aluminium toxicity, Aluminium-sensitive rice mutant, Germination, Rice, Root growth, Wheat",
author = "Satoshi Kikui and Takayuki Sasaki and Masahiko Maekawa and Akio Miyao and Hirohiko Hirochika and Hideaki Matsumoto and Yoko Yamamoto",
year = "2005",
month = "9",
doi = "10.1016/j.jinorgbio.2005.06.031",
language = "English",
volume = "99",
pages = "1837--1844",
journal = "Journal of Inorganic Biochemistry",
issn = "0162-0134",
publisher = "Elsevier Inc.",
number = "9 SPEC. ISS.",

}

TY - JOUR

T1 - Physiological and genetic analyses of aluminium tolerance in rice, focusing on root growth during germination

AU - Kikui, Satoshi

AU - Sasaki, Takayuki

AU - Maekawa, Masahiko

AU - Miyao, Akio

AU - Hirochika, Hirohiko

AU - Matsumoto, Hideaki

AU - Yamamoto, Yoko

PY - 2005/9

Y1 - 2005/9

N2 - Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.

AB - Aluminium (Al) ion limits root growth of plants in acidic soils, and rice exhibits the highest level of Al-tolerance among graminous crops. To elucidate Al-tolerance mechanisms in rice, response to Al was compared between rice (Oryza sativa L., cv. Nipponbare) and wheat (Triticum aestivum L., cv. ET8), focusing on seminal root growth at seedling stage and germination stage. At seedling stage, rice and wheat were similarly sensitive to Al in both dose- and time-dependent manner during a 24-h Al exposure. On the contrary, at germination stage, rice was more tolerant to Al than wheat, and wheat roots displayed the loss of plasma membrane integrity more extensively than rice. A rice mutant exhibiting Al hypersensitivity at germination stage was obtained by screening 42,840 R2 progeny derived from the regenerated R0 plants of Nipponbare and thereafter confirmation of the mutant phenotype in R 3 progeny. At germination stage, root growth of the mutant was strongly inhibited in the presence of Al but not in the absence of Al. However, at seedling stage, root growth of the mutant and wild type was similarly tolerant to Al. Taken together, we conclude that rice possesses Al-tolerant function that is under genetic control and specifically operates for root growth at germination stage, making rice more tolerant to Al than wheat.

KW - Aluminium toxicity

KW - Aluminium-sensitive rice mutant

KW - Germination

KW - Rice

KW - Root growth

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=24344456474&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24344456474&partnerID=8YFLogxK

U2 - 10.1016/j.jinorgbio.2005.06.031

DO - 10.1016/j.jinorgbio.2005.06.031

M3 - Article

VL - 99

SP - 1837

EP - 1844

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

SN - 0162-0134

IS - 9 SPEC. ISS.

ER -