Abstract
A plasmid library of restriction DNA fragments of a Plutella xylostella granulovirus (PxGV) was constructed, which covered all 9 EcoRI and all 19 HindIII fragments, and 18 of 20 PstI fragments. Restriction endonuclease analysis and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for construction of a physical map of PxGV DNA for ApaI, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimated to be 102.2 kb. Location of the left-most DNA fragment on the physical map that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous regions interspersed on the genome were also determined by DNA-DNA hybridization analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 45-51 |
| Number of pages | 7 |
| Journal | Applied Entomology and Zoology |
| Volume | 35 |
| Issue number | 1 |
| Publication status | Published - Feb 2000 |
| Externally published | Yes |
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Keywords
- Granulovirus
- Physical map
- Plutella xylostella
ASJC Scopus subject areas
- Insect Science
Cite this
Physical map of a Plutella xylostella granulovirus genome. / Hashimoto, Yoshifumi; Hayashi, Kana; Hayakawa, Tohru; Ueno, Yasumasa; Shimojo, Ei Ichi; Kondo, Atsushi; Miyasono, Minoru; Sano, Yoshitaka; Matsumoto, Tsuguo; Granados, Robert R.
In: Applied Entomology and Zoology, Vol. 35, No. 1, 02.2000, p. 45-51.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physical map of a Plutella xylostella granulovirus genome
AU - Hashimoto, Yoshifumi
AU - Hayashi, Kana
AU - Hayakawa, Tohru
AU - Ueno, Yasumasa
AU - Shimojo, Ei Ichi
AU - Kondo, Atsushi
AU - Miyasono, Minoru
AU - Sano, Yoshitaka
AU - Matsumoto, Tsuguo
AU - Granados, Robert R.
PY - 2000/2
Y1 - 2000/2
N2 - A plasmid library of restriction DNA fragments of a Plutella xylostella granulovirus (PxGV) was constructed, which covered all 9 EcoRI and all 19 HindIII fragments, and 18 of 20 PstI fragments. Restriction endonuclease analysis and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for construction of a physical map of PxGV DNA for ApaI, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimated to be 102.2 kb. Location of the left-most DNA fragment on the physical map that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous regions interspersed on the genome were also determined by DNA-DNA hybridization analysis.
AB - A plasmid library of restriction DNA fragments of a Plutella xylostella granulovirus (PxGV) was constructed, which covered all 9 EcoRI and all 19 HindIII fragments, and 18 of 20 PstI fragments. Restriction endonuclease analysis and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for construction of a physical map of PxGV DNA for ApaI, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimated to be 102.2 kb. Location of the left-most DNA fragment on the physical map that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous regions interspersed on the genome were also determined by DNA-DNA hybridization analysis.
KW - Granulovirus
KW - Physical map
KW - Plutella xylostella
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UR - http://www.scopus.com/inward/citedby.url?scp=0034340024&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0034340024
VL - 35
SP - 45
EP - 51
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
SN - 0003-6862
IS - 1
ER -