Physical map of a Plutella xylostella granulovirus genome

Yoshifumi Hashimoto, Kana Hayashi, Tohru Hayakawa, Yasumasa Ueno, Ei Ichi Shimojo, Atsushi Kondo, Minoru Miyasono, Yoshitaka Sano, Tsuguo Matsumoto, Robert R. Granados

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A plasmid library of restriction DNA fragments of a Plutella xylostella granulovirus (PxGV) was constructed, which covered all 9 EcoRI and all 19 HindIII fragments, and 18 of 20 PstI fragments. Restriction endonuclease analysis and DNA-DNA hybridization analysis of the cloned viral DNA fragments in the library and the restricted viral DNA fragments were carried out for construction of a physical map of PxGV DNA for ApaI, BamHI, BglII, EcoRI, HindIII, KpnI, PstI, SacII, XbaI and XhoI. The size of PxGV genome was estimated to be 102.2 kb. Location of the left-most DNA fragment on the physical map that contained granulin gene was determined by hybridization using a DNA probe of granulin gene of Trichoplusia ni granulovirus. Four homologous regions interspersed on the genome were also determined by DNA-DNA hybridization analysis.

Original languageEnglish
Pages (from-to)45-51
Number of pages7
JournalApplied Entomology and Zoology
Volume35
Issue number1
DOIs
Publication statusPublished - Feb 2000
Externally publishedYes

Keywords

  • Granulovirus
  • Physical map
  • Plutella xylostella

ASJC Scopus subject areas

  • Insect Science

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  • Cite this

    Hashimoto, Y., Hayashi, K., Hayakawa, T., Ueno, Y., Shimojo, E. I., Kondo, A., Miyasono, M., Sano, Y., Matsumoto, T., & Granados, R. R. (2000). Physical map of a Plutella xylostella granulovirus genome. Applied Entomology and Zoology, 35(1), 45-51. https://doi.org/10.1303/aez.2000.45