Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis

Kazusato Oikawa, Shigeru Matsunaga, Shoji Mano, Maki Kondo, Kenji Yamada, Makoto Hayashi, Takatoshi Kagawa, Akeo Kadota, Wataru Sakamoto, Shoichi Higashi, Masakatsu Watanabe, Toshiaki Mitsui, Akinori Shigemasa, Takanori Iino, Yoichiroh Hosokawa, Mikio Nishimura

    Research output: Contribution to journalArticlepeer-review

    71 Citations (Scopus)

    Abstract

    Life on earth relies upon photosynthesis, which consumes carbon dioxide and generates oxygen and carbohydrates. Photosynthesis is sustained by a dynamic environment within the plant cell involving numerous organelles with cytoplasmic streaming. Physiological studies of chloroplasts, mitochondria and peroxisomes show that these organelles actively communicate during photorespiration, a process by which by-products produced by photosynthesis are salvaged. Nevertheless, the mechanisms enabling efficient exchange of metabolites have not been clearly defined. We found that peroxisomes along chloroplasts changed shape from spherical to elliptical and their interaction area increased during photorespiration. We applied a recent femtosecond laser technology to analyse adhesion between the organelles inside palisade mesophyll cells of Arabidopsis leaves and succeeded in estimating their physical interactions under different environmental conditions. This is the first application of this estimation method within living cells. Our findings suggest that photosynthetic-dependent interactions play a critical role in ensuring efficient metabolite flow during photorespiration.

    Original languageEnglish
    Article number15035
    JournalNature Plants
    Volume1
    DOIs
    Publication statusPublished - Mar 30 2015

    ASJC Scopus subject areas

    • Plant Science

    Fingerprint

    Dive into the research topics of 'Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis'. Together they form a unique fingerprint.

    Cite this