TY - JOUR
T1 - Physical, biochemical and genetic characterization of enterocin CE5-1 produced by enterococcus faecium CE5-1 isolated from Thai indigenous chicken intestinal tract
AU - Saelim, Kraiyot
AU - Kaewsuwan, Sireewan
AU - Tani, Akio
AU - Maneerat, Suppasil
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Enterocin CE5-1 produced by Enterococcus faecium CE5-1 isolated from the chicken gastrointestinal tract was active in the wide range of pH 2-10 and temperature 30-100°C and sensitive to proteolytic enzymes and α-amylase. It remained active after storage at -20°C for 2 months. Moreover, enterocin CE5-1 showed antibacterial activity against lactobacilli, bacilli, listeria, staphylococci and enterococci, especially antibiotic-resistant enterococci. In vitro study of enterocin CE5-1 decreased the population of Ent. faecalis VanB from 6.03 to 4.03 log CFU/ml. The lethal mode of action of enterocin CE5-1 appeared to be pore and filament formation in the cell wall. PCR sequencing analysis revealed the presence of two open reading frames (ORFs), containing enterocin CE5-1 (entCE5-1) and enterocin immunity (entI) gene. Therefore, enterocin CE5-1 from Ent. faecium CE5-1 could possibly be used as an antimicrobial agent to control foodborne pathogen, spoilage bacteria and antibiotic-resistant enterococci in foods, feeds and the environments.
AB - Enterocin CE5-1 produced by Enterococcus faecium CE5-1 isolated from the chicken gastrointestinal tract was active in the wide range of pH 2-10 and temperature 30-100°C and sensitive to proteolytic enzymes and α-amylase. It remained active after storage at -20°C for 2 months. Moreover, enterocin CE5-1 showed antibacterial activity against lactobacilli, bacilli, listeria, staphylococci and enterococci, especially antibiotic-resistant enterococci. In vitro study of enterocin CE5-1 decreased the population of Ent. faecalis VanB from 6.03 to 4.03 log CFU/ml. The lethal mode of action of enterocin CE5-1 appeared to be pore and filament formation in the cell wall. PCR sequencing analysis revealed the presence of two open reading frames (ORFs), containing enterocin CE5-1 (entCE5-1) and enterocin immunity (entI) gene. Therefore, enterocin CE5-1 from Ent. faecium CE5-1 could possibly be used as an antimicrobial agent to control foodborne pathogen, spoilage bacteria and antibiotic-resistant enterococci in foods, feeds and the environments.
KW - Antibiotic-resistant enterococci
KW - Chicken
KW - Enterocin
KW - Enterococcus faecium
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M3 - Article
AN - SCOPUS:84938859957
VL - 37
SP - 299
EP - 307
JO - Songklanakarin Journal of Science and Technology
JF - Songklanakarin Journal of Science and Technology
SN - 0125-3395
IS - 3
ER -