Phylogeographic characteristics of hypervariable regions in the mitochondrial genome of a cosmopolitan, bloom-forming raphidophyte, Heterosigma akashiwo

Research output: Contribution to journalArticle

Abstract

The global distribution of phytoplankton is defined by many events, including long-term evolutionary processes and shorter time span processes (e.g., global climate change). Furthermore, human-assisted, unintentional dispersion, including the transport of live fish and spat for aquaculture, and transfer of aquatic microorganisms contained in ship ballast water, may aid the spread of phytoplankton. To understand the phylogeographic history of a species, the development of useful molecular markers is crucial. We previously reported a hypervariable mitochondrial gene in the cosmopolitan bloom-forming alga, Heterosigma akashiwo. In this study, we identified two additional hypervariable segments in the H. akashiwo mitochondrial genome, one a protein coding sequence, and the other an intergenic region, by comparing the whole mitogenomes of strains obtained from various geographic origins. Interestingly, the newly identified hypervariable protein coding sequence was a paralogue of the previously identified gene, and both sequences showed tendencies to correlate with latitude of geographic origin. However, the hypervariable intergenic sequence did not show a clear correlation with origin. Our results demonstrated that the protein coding sequences may serve as useful tools for understanding the phylogeographic history of H. akashiwo, and they may crucially function in adaptation of the species to the environment.

Original languageEnglish
JournalJournal of Phycology
DOIs
Publication statusPublished - Jan 1 2019

Fingerprint

algal bloom
genome
intergenic DNA
algae
provenance
protein
phytoplankton
ballast water
history
proteins
gene
spit
ships
aquaculture
global climate
genes
microorganism
alga
climate change
microorganisms

Keywords

  • harmful algal bloom
  • Heterosigma akashiwo
  • mitochondrial genome
  • mitochondrial hypervariable sequences
  • phylogeography

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

@article{49afa69bc20247178faf07de839dfd85,
title = "Phylogeographic characteristics of hypervariable regions in the mitochondrial genome of a cosmopolitan, bloom-forming raphidophyte, Heterosigma akashiwo",
abstract = "The global distribution of phytoplankton is defined by many events, including long-term evolutionary processes and shorter time span processes (e.g., global climate change). Furthermore, human-assisted, unintentional dispersion, including the transport of live fish and spat for aquaculture, and transfer of aquatic microorganisms contained in ship ballast water, may aid the spread of phytoplankton. To understand the phylogeographic history of a species, the development of useful molecular markers is crucial. We previously reported a hypervariable mitochondrial gene in the cosmopolitan bloom-forming alga, Heterosigma akashiwo. In this study, we identified two additional hypervariable segments in the H. akashiwo mitochondrial genome, one a protein coding sequence, and the other an intergenic region, by comparing the whole mitogenomes of strains obtained from various geographic origins. Interestingly, the newly identified hypervariable protein coding sequence was a paralogue of the previously identified gene, and both sequences showed tendencies to correlate with latitude of geographic origin. However, the hypervariable intergenic sequence did not show a clear correlation with origin. Our results demonstrated that the protein coding sequences may serve as useful tools for understanding the phylogeographic history of H. akashiwo, and they may crucially function in adaptation of the species to the environment.",
keywords = "harmful algal bloom, Heterosigma akashiwo, mitochondrial genome, mitochondrial hypervariable sequences, phylogeography",
author = "Shoko Ueki",
year = "2019",
month = "1",
day = "1",
doi = "10.1111/jpy.12868",
language = "English",
journal = "Journal of Phycology",
issn = "0022-3646",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Phylogeographic characteristics of hypervariable regions in the mitochondrial genome of a cosmopolitan, bloom-forming raphidophyte, Heterosigma akashiwo

AU - Ueki, Shoko

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The global distribution of phytoplankton is defined by many events, including long-term evolutionary processes and shorter time span processes (e.g., global climate change). Furthermore, human-assisted, unintentional dispersion, including the transport of live fish and spat for aquaculture, and transfer of aquatic microorganisms contained in ship ballast water, may aid the spread of phytoplankton. To understand the phylogeographic history of a species, the development of useful molecular markers is crucial. We previously reported a hypervariable mitochondrial gene in the cosmopolitan bloom-forming alga, Heterosigma akashiwo. In this study, we identified two additional hypervariable segments in the H. akashiwo mitochondrial genome, one a protein coding sequence, and the other an intergenic region, by comparing the whole mitogenomes of strains obtained from various geographic origins. Interestingly, the newly identified hypervariable protein coding sequence was a paralogue of the previously identified gene, and both sequences showed tendencies to correlate with latitude of geographic origin. However, the hypervariable intergenic sequence did not show a clear correlation with origin. Our results demonstrated that the protein coding sequences may serve as useful tools for understanding the phylogeographic history of H. akashiwo, and they may crucially function in adaptation of the species to the environment.

AB - The global distribution of phytoplankton is defined by many events, including long-term evolutionary processes and shorter time span processes (e.g., global climate change). Furthermore, human-assisted, unintentional dispersion, including the transport of live fish and spat for aquaculture, and transfer of aquatic microorganisms contained in ship ballast water, may aid the spread of phytoplankton. To understand the phylogeographic history of a species, the development of useful molecular markers is crucial. We previously reported a hypervariable mitochondrial gene in the cosmopolitan bloom-forming alga, Heterosigma akashiwo. In this study, we identified two additional hypervariable segments in the H. akashiwo mitochondrial genome, one a protein coding sequence, and the other an intergenic region, by comparing the whole mitogenomes of strains obtained from various geographic origins. Interestingly, the newly identified hypervariable protein coding sequence was a paralogue of the previously identified gene, and both sequences showed tendencies to correlate with latitude of geographic origin. However, the hypervariable intergenic sequence did not show a clear correlation with origin. Our results demonstrated that the protein coding sequences may serve as useful tools for understanding the phylogeographic history of H. akashiwo, and they may crucially function in adaptation of the species to the environment.

KW - harmful algal bloom

KW - Heterosigma akashiwo

KW - mitochondrial genome

KW - mitochondrial hypervariable sequences

KW - phylogeography

UR - http://www.scopus.com/inward/record.url?scp=85068087282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85068087282&partnerID=8YFLogxK

U2 - 10.1111/jpy.12868

DO - 10.1111/jpy.12868

M3 - Article

C2 - 31032911

AN - SCOPUS:85068087282

JO - Journal of Phycology

JF - Journal of Phycology

SN - 0022-3646

ER -