Abstract
We previously showed that H. secalinum and H. capense are allotetraploids carrying the Xa genome of H. marinum and the I genome of an unidentified diploid species. In this study, intraspecific variation in each tetraploid species was investigated with regard to intergenomic translocations and chromosomal distribution of rDNA sites. Genomic in situ hybridization revealed that three H. secalinum accessions examined did not carry intergenomic translocations, but that two of three H. capsense accessions analyzed carried a pair of inter- genomic translocations. Multicolour fluorescence in situ hybridization showed that H. secalinum included two types of rDNA pattern differing in the presence or absence of an extra 5S rDNA site in a submetacentric chromo- some pair of the Xa-genome origin. The extra 5S rDNA site was found in all H. capense accessions examined. This 5S rDNA site is characteristic of H. marinum ssp. gussoneanum, but is absent in ssp. marinum. Poly- morphisms in the 5S rDNA site infer that H. secalinum included two types, one having ssp. gussoneanum 2x and the other having ssp. marinum, as the Xa-genome donor. We conclude that H. capense originated from a limited number of H. secalinum accessions introduced probably through migrating birds to South Africa.
| Original language | English |
|---|---|
| Pages (from-to) | 589-594 |
| Number of pages | 6 |
| Journal | Breeding Science |
| Volume | 59 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- Chromosome
- FISH
- GISH
- Nucleolus organizer region
- Translocation
- Wild barley
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Plant Science
Cite this
Phylogeny of two tetraploid Hordeum species, H. secalinum and H. capense inferred from physical mapping of 5S and 18S-25S rDNA. / Taketa, Shin; Nakauchi, Yasuko; von Bothmer, Roland.
In: Breeding Science, Vol. 59, No. 5, 2009, p. 589-594.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phylogeny of two tetraploid Hordeum species, H. secalinum and H. capense inferred from physical mapping of 5S and 18S-25S rDNA
AU - Taketa, Shin
AU - Nakauchi, Yasuko
AU - von Bothmer, Roland
PY - 2009
Y1 - 2009
N2 - We previously showed that H. secalinum and H. capense are allotetraploids carrying the Xa genome of H. marinum and the I genome of an unidentified diploid species. In this study, intraspecific variation in each tetraploid species was investigated with regard to intergenomic translocations and chromosomal distribution of rDNA sites. Genomic in situ hybridization revealed that three H. secalinum accessions examined did not carry intergenomic translocations, but that two of three H. capsense accessions analyzed carried a pair of inter- genomic translocations. Multicolour fluorescence in situ hybridization showed that H. secalinum included two types of rDNA pattern differing in the presence or absence of an extra 5S rDNA site in a submetacentric chromo- some pair of the Xa-genome origin. The extra 5S rDNA site was found in all H. capense accessions examined. This 5S rDNA site is characteristic of H. marinum ssp. gussoneanum, but is absent in ssp. marinum. Poly- morphisms in the 5S rDNA site infer that H. secalinum included two types, one having ssp. gussoneanum 2x and the other having ssp. marinum, as the Xa-genome donor. We conclude that H. capense originated from a limited number of H. secalinum accessions introduced probably through migrating birds to South Africa.
AB - We previously showed that H. secalinum and H. capense are allotetraploids carrying the Xa genome of H. marinum and the I genome of an unidentified diploid species. In this study, intraspecific variation in each tetraploid species was investigated with regard to intergenomic translocations and chromosomal distribution of rDNA sites. Genomic in situ hybridization revealed that three H. secalinum accessions examined did not carry intergenomic translocations, but that two of three H. capsense accessions analyzed carried a pair of inter- genomic translocations. Multicolour fluorescence in situ hybridization showed that H. secalinum included two types of rDNA pattern differing in the presence or absence of an extra 5S rDNA site in a submetacentric chromo- some pair of the Xa-genome origin. The extra 5S rDNA site was found in all H. capense accessions examined. This 5S rDNA site is characteristic of H. marinum ssp. gussoneanum, but is absent in ssp. marinum. Poly- morphisms in the 5S rDNA site infer that H. secalinum included two types, one having ssp. gussoneanum 2x and the other having ssp. marinum, as the Xa-genome donor. We conclude that H. capense originated from a limited number of H. secalinum accessions introduced probably through migrating birds to South Africa.
KW - Chromosome
KW - FISH
KW - GISH
KW - Nucleolus organizer region
KW - Translocation
KW - Wild barley
UR - http://www.scopus.com/inward/record.url?scp=72549088745&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=72549088745&partnerID=8YFLogxK
U2 - 10.1270/jsbbs.59.589
DO - 10.1270/jsbbs.59.589
M3 - Article
AN - SCOPUS:72549088745
VL - 59
SP - 589
EP - 594
JO - Breeding Science
JF - Breeding Science
SN - 1344-7610
IS - 5
ER -