Phylogenetic characterization of a new thermoacidophilic bacterium isolated from hot springs in Japan

Akira Hiraishi, Kenji Inagaki, Yasuhide Tanimoto, Mitsuru Iwasaki, Noriaki Kishimoto, Hidehiko Tanaka

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27 Citations (Scopus)


Three strains of thermophilic-acidophilic bacteria isolated previously from different hot springs in Japan were characterized by molecular genetic methods. The strategy taken involved PCR amplification, sequencing and restriction pattern analysis of 16S rDNA, 16S-23S rDNA spacer polymorphism analysis and genomic DNA-DNA hybridization. A phylogenetic analysis based on 16S rDNA sequences showed that the new thermoacidophilic isolates formed a genetically coherent group at the species level and fell into a major cluster together with members of the genera Alicyclobacillus and Sulfobacillus with A. acidocaldarius and A. acidoterrestris as their closest relatives. The levels of binary sequence similarity between the isolates and the two Alicyclobacillus species were 97.6 to 97.9%, values considered low enough to warrant placement of the isolates in a distinct species of the genus Alicyclobacillus. The 16S rDNA restriction pattern analysis, but not 16S-23S rDNA spacer polymorphism analysis, was useful for differentiating the isolates from the established Alicyclobacillus species. DNA-DNA hybridization assays demonstrated a distinct phylogenetic position of our isolates as a genospecies within the genus Alicyclobacillus. On the basis of these results, the thermoacidophilic isolates should be classified into a new species of Alicyclobacillus. The results of this study suggest that this new genospecies of Alicyclobacillus is widely distributed in hot springs in Japan.

Original languageEnglish
Pages (from-to)295-304
Number of pages10
JournalJournal of General and Applied Microbiology
Issue number5
Publication statusPublished - 1997


  • 16S rDNA
  • Alicyclobacillus
  • Hot spring
  • Phylogeny
  • Thermoacidophiles

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology


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