Photosystem II complex in vivo is a monomer

Takeshi Takahashi, Natsuko Inoue-Kashino, Shin Ichiro Ozawa, Yuichiro Takahashi, Yashuhiro Kashino, Kazuhiko Satoh

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Photosystem II (PS II) complexes are membrane protein complexes that are composed of >20 distinct subunit proteins. Similar to many other membrane protein complexes, two PS II complexes are believed to form a homo-dimer whose molecular mass is ∼650 kDa. Contrary to this well known concept, we propose that the functional form of PS II in vivo is a monomer, based on the following observations. Deprivation of lipids caused the conversion of PS II from a monomeric form to a dimeric form. Only a monomeric PS II Was detected in solubilized cyanobacterial and red algal thylakoids using blue-native polyacrylamide gel electrophoresis. Furthermore, energy transfer between PS II units, which was observed in the purified dimeric PS II, was not detected in vivo. Our proposal will lead to a re-evaluation of many crystallographic models of membrane protein complexes in terms of their oligomerization status.

Original languageEnglish
Pages (from-to)15598-15606
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number23
DOIs
Publication statusPublished - Jun 5 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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