Photosystem II complex in vivo is a monomer

Takeshi Takahashi, Natsuko Inoue-Kashino, Shinichiro Ozawa, Yuichiro Takahashi, Yashuhiro Kashino, Kazuhiko Satoh

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Photosystem II (PS II) complexes are membrane protein complexes that are composed of >20 distinct subunit proteins. Similar to many other membrane protein complexes, two PS II complexes are believed to form a homo-dimer whose molecular mass is ∼650 kDa. Contrary to this well known concept, we propose that the functional form of PS II in vivo is a monomer, based on the following observations. Deprivation of lipids caused the conversion of PS II from a monomeric form to a dimeric form. Only a monomeric PS II Was detected in solubilized cyanobacterial and red algal thylakoids using blue-native polyacrylamide gel electrophoresis. Furthermore, energy transfer between PS II units, which was observed in the purified dimeric PS II, was not detected in vivo. Our proposal will lead to a re-evaluation of many crystallographic models of membrane protein complexes in terms of their oligomerization status.

Original languageEnglish
Pages (from-to)15598-15606
Number of pages9
JournalJournal of Biological Chemistry
Volume284
Issue number23
DOIs
Publication statusPublished - Jun 5 2009

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Photosystem II Protein Complex
Monomers
Membrane Proteins
Native Polyacrylamide Gel Electrophoresis
Oligomerization
Thylakoids
Energy Transfer
Protein Subunits
Molecular mass
Electrophoresis
Dimers
Energy transfer
Lipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Takahashi, T., Inoue-Kashino, N., Ozawa, S., Takahashi, Y., Kashino, Y., & Satoh, K. (2009). Photosystem II complex in vivo is a monomer. Journal of Biological Chemistry, 284(23), 15598-15606. https://doi.org/10.1074/jbc.M109.000372

Photosystem II complex in vivo is a monomer. / Takahashi, Takeshi; Inoue-Kashino, Natsuko; Ozawa, Shinichiro; Takahashi, Yuichiro; Kashino, Yashuhiro; Satoh, Kazuhiko.

In: Journal of Biological Chemistry, Vol. 284, No. 23, 05.06.2009, p. 15598-15606.

Research output: Contribution to journalArticle

Takahashi, T, Inoue-Kashino, N, Ozawa, S, Takahashi, Y, Kashino, Y & Satoh, K 2009, 'Photosystem II complex in vivo is a monomer', Journal of Biological Chemistry, vol. 284, no. 23, pp. 15598-15606. https://doi.org/10.1074/jbc.M109.000372
Takahashi, Takeshi ; Inoue-Kashino, Natsuko ; Ozawa, Shinichiro ; Takahashi, Yuichiro ; Kashino, Yashuhiro ; Satoh, Kazuhiko. / Photosystem II complex in vivo is a monomer. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 23. pp. 15598-15606.
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