Photosystem II complex in vivo is a monomer

Takeshi Takahashi, Natsuko Inoue-Kashino, Shin Ichiro Ozawa, Yuichiro Takahashi, Yashuhiro Kashino, Kazuhiko Satoh

    Research output: Contribution to journalArticlepeer-review

    45 Citations (Scopus)

    Abstract

    Photosystem II (PS II) complexes are membrane protein complexes that are composed of >20 distinct subunit proteins. Similar to many other membrane protein complexes, two PS II complexes are believed to form a homo-dimer whose molecular mass is ∼650 kDa. Contrary to this well known concept, we propose that the functional form of PS II in vivo is a monomer, based on the following observations. Deprivation of lipids caused the conversion of PS II from a monomeric form to a dimeric form. Only a monomeric PS II Was detected in solubilized cyanobacterial and red algal thylakoids using blue-native polyacrylamide gel electrophoresis. Furthermore, energy transfer between PS II units, which was observed in the purified dimeric PS II, was not detected in vivo. Our proposal will lead to a re-evaluation of many crystallographic models of membrane protein complexes in terms of their oligomerization status.

    Original languageEnglish
    Pages (from-to)15598-15606
    Number of pages9
    JournalJournal of Biological Chemistry
    Volume284
    Issue number23
    DOIs
    Publication statusPublished - Jun 5 2009

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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