Photosystem II and the unique role of bicarbonate: A historical perspective

Dmitriy Shevela, Julian J. Eaton-Rye, Jian-Ren Shen, Govindjee

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

In photosynthesis, cyanobacteria, algae and plants fix carbon dioxide (CO2) into carbohydrates; this is necessary to support life on Earth. Over 50 years ago, Otto Heinrich Warburg discovered a unique stimulatory role of CO2 in the Hill reaction (i.e., O2 evolution accompanied by reduction of an artificial electron acceptor), which, obviously, does not include any carbon fixation pathway; Warburg used this discovery to support his idea that O2 in photosynthesis originates in CO 2. During the 1960s, a large number of researchers attempted to decipher this unique phenomenon, with limited success. In the 1970s, Alan Stemler, in Govindjee's lab, perfected methods to get highly reproducible results, and observed, among other things, that the turnover of Photosystem II (PSII) was stimulated by bicarbonate ions (hydrogen carbonate): the effect would be on the donor or the acceptor, or both sides of PSII. In 1975, Thomas Wydrzynski, also in Govindjee's lab, discovered that there was a definite bicarbonate effect on the electron acceptor (the plastoquinone) side of PSII. The most recent 1.9 Å crystal structure of PSII, unequivocally shows HCO3 - bound to the non-heme iron that sits in-between the bound primary quinone electron acceptor, QA, and the secondary quinone electron acceptor QB. In this review, we focus on the historical development of our understanding of this unique bicarbonate effect on the electron acceptor side of PSII, and its mechanism as obtained by biochemical, biophysical and molecular biological approaches in many laboratories around the World. We suggest an atomic level model in which HCO3 -/CO3 2 - plays a key role in the protonation of the reduced QB. In addition, we make comments on the role of bicarbonate on the donor side of PSII, as has been extensively studied in the labs of Alan Stemler (USA) and Vyacheslav Klimov (Russia). We end this review by discussing the uniqueness of bicarbonate's role in oxygenic photosynthesis and its role in the evolutionary development of O 2-evolving PSII. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: from Natural to Artificial.

Original languageEnglish
Pages (from-to)1134-1151
Number of pages18
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1817
Issue number8
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Photosystem II Protein Complex
Bicarbonates
Photosynthesis
Electrons
Plastoquinone
Carbon Cycle
Protonation
Russia
Cyanobacteria
Carbon Monoxide
Algae
Carbon Dioxide
Sustainable development
Carbon
Iron
Crystal structure
Earth (planet)
Carbohydrates
Research Personnel
Research

Keywords

  • Bicarbonate (hydrogen carbonate) effect
  • Electron transport
  • O evolution
  • Photosystem II
  • Protonation reaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Photosystem II and the unique role of bicarbonate : A historical perspective. / Shevela, Dmitriy; Eaton-Rye, Julian J.; Shen, Jian-Ren; Govindjee.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1817, No. 8, 08.2012, p. 1134-1151.

Research output: Contribution to journalArticle

Shevela, Dmitriy ; Eaton-Rye, Julian J. ; Shen, Jian-Ren ; Govindjee. / Photosystem II and the unique role of bicarbonate : A historical perspective. In: Biochimica et Biophysica Acta - Bioenergetics. 2012 ; Vol. 1817, No. 8. pp. 1134-1151.
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