Abstract
An oxygen-evolving photosynthetic reaction center complex (PSII) was adsorbed into nanopores in SBA, a mesoporous silica compound. We purified the dimer of PSII complex from a thermophilic cyanobacterium, Thermosynechococcus vulcanus, which grows optimally at 57 °C. The thermally stable PSII dimeric complex has a diameter of 20 nm and a molecular mass of 756 kDa and binds more than 60 chlorophylls. The SBA particles, with average internal pore diameters of 15 nm (SBA 15) and 23 nm (SBA 23), adsorbed 4.7 and 15 mg of PSII/g SBA, respectively. Measurement with a confocal laser-scanning microscope indicated the adsorption of PSII to the surface and the inner space of the SBA 23 particles, indicating the adsorption of PSII into the 23 nm silica nanopores. PSII did not bind to the inner pores of SBA 15. PSII bound to SBA 23 showed the high and stable activity of a photosynthetic oxygen-evolving reaction, indicating the light-driven electron transport from water to the quinone molecules added in the outer medium. The PSII-SBA conjugate can be a new material for photosensors and artificial photosynthetic systems.
| Original language | English |
|---|---|
| Pages (from-to) | 705-713 |
| Number of pages | 9 |
| Journal | Langmuir |
| Volume | 27 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jan 18 2011 |
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ASJC Scopus subject areas
- Electrochemistry
- Condensed Matter Physics
- Surfaces and Interfaces
- Materials Science(all)
- Spectroscopy
Cite this
Photosynthetic oxygen evolution in mesoporous silica material : Adsorption of photosystem II reaction center complex into 23 nm nanopores in SBA. / Noji, Tomoyasu; Kamidaki, Chihiro; Kawakami, Keisuke; Shen, Jian-Ren; Kajino, Tsutomu; Fukushima, Yoshiaki; Sekitoh, Takeshi; Itoh, Shigeru.
In: Langmuir, Vol. 27, No. 2, 18.01.2011, p. 705-713.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Photosynthetic oxygen evolution in mesoporous silica material
T2 - Adsorption of photosystem II reaction center complex into 23 nm nanopores in SBA
AU - Noji, Tomoyasu
AU - Kamidaki, Chihiro
AU - Kawakami, Keisuke
AU - Shen, Jian-Ren
AU - Kajino, Tsutomu
AU - Fukushima, Yoshiaki
AU - Sekitoh, Takeshi
AU - Itoh, Shigeru
PY - 2011/1/18
Y1 - 2011/1/18
N2 - An oxygen-evolving photosynthetic reaction center complex (PSII) was adsorbed into nanopores in SBA, a mesoporous silica compound. We purified the dimer of PSII complex from a thermophilic cyanobacterium, Thermosynechococcus vulcanus, which grows optimally at 57 °C. The thermally stable PSII dimeric complex has a diameter of 20 nm and a molecular mass of 756 kDa and binds more than 60 chlorophylls. The SBA particles, with average internal pore diameters of 15 nm (SBA 15) and 23 nm (SBA 23), adsorbed 4.7 and 15 mg of PSII/g SBA, respectively. Measurement with a confocal laser-scanning microscope indicated the adsorption of PSII to the surface and the inner space of the SBA 23 particles, indicating the adsorption of PSII into the 23 nm silica nanopores. PSII did not bind to the inner pores of SBA 15. PSII bound to SBA 23 showed the high and stable activity of a photosynthetic oxygen-evolving reaction, indicating the light-driven electron transport from water to the quinone molecules added in the outer medium. The PSII-SBA conjugate can be a new material for photosensors and artificial photosynthetic systems.
AB - An oxygen-evolving photosynthetic reaction center complex (PSII) was adsorbed into nanopores in SBA, a mesoporous silica compound. We purified the dimer of PSII complex from a thermophilic cyanobacterium, Thermosynechococcus vulcanus, which grows optimally at 57 °C. The thermally stable PSII dimeric complex has a diameter of 20 nm and a molecular mass of 756 kDa and binds more than 60 chlorophylls. The SBA particles, with average internal pore diameters of 15 nm (SBA 15) and 23 nm (SBA 23), adsorbed 4.7 and 15 mg of PSII/g SBA, respectively. Measurement with a confocal laser-scanning microscope indicated the adsorption of PSII to the surface and the inner space of the SBA 23 particles, indicating the adsorption of PSII into the 23 nm silica nanopores. PSII did not bind to the inner pores of SBA 15. PSII bound to SBA 23 showed the high and stable activity of a photosynthetic oxygen-evolving reaction, indicating the light-driven electron transport from water to the quinone molecules added in the outer medium. The PSII-SBA conjugate can be a new material for photosensors and artificial photosynthetic systems.
UR - http://www.scopus.com/inward/record.url?scp=78651277184&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78651277184&partnerID=8YFLogxK
U2 - 10.1021/la1032916
DO - 10.1021/la1032916
M3 - Article
C2 - 21171572
AN - SCOPUS:78651277184
VL - 27
SP - 705
EP - 713
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 2
ER -