Photosensitizing carrier proteins for photoinducible RNA interference

Yuka Matsushita-Ishiodori, Rina Kuwabara, Hiroyuki Sakakoshi, Tamaki Endoh, Takashi Ohtsuki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

Original languageEnglish
Pages (from-to)2222-2226
Number of pages5
JournalBioconjugate Chemistry
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 16 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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  • Cite this

    Matsushita-Ishiodori, Y., Kuwabara, R., Sakakoshi, H., Endoh, T., & Ohtsuki, T. (2011). Photosensitizing carrier proteins for photoinducible RNA interference. Bioconjugate Chemistry, 22(11), 2222-2226. https://doi.org/10.1021/bc200095a