Photosensitizing carrier proteins for photoinducible RNA interference

Yuka Matsushita-Ishiodori, Rina Kuwabara, Hiroyuki Sakakoshi, Tamaki Endoh, Takashi Ohtsuki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

RNA interference (RNAi) is being widely explored as a tool in functional genomics and tissue engineering, and in the therapy of intractable diseases, including cancer and neurodegenerative diseases. Recently, we developed a photoinducible RNAi method using photosensitizing carrier proteins, named CLIP-RNAi (CPP-linked RBP-mediated RNA internalization and photoinduced RNAi). Novel carrier proteins were designed for this study to establish a highly efficient delivery system for small interfering RNA (siRNA) or short hairpin RNA (shRNA) and to demonstrate light-dependent gene silencing. In addition, the results suggested that the dissociation of the siRNA (or shRNA) from carrier proteins in the cytoplasm is a critical event in CLIP-RNAi-mediated gene silencing.

Original languageEnglish
Pages (from-to)2222-2226
Number of pages5
JournalBioconjugate Chemistry
Volume22
Issue number11
DOIs
Publication statusPublished - Nov 16 2011

Fingerprint

RNA Interference
RNA
Carrier Proteins
Small Interfering RNA
Gene Silencing
Genes
Neurodegenerative diseases
Tissue Engineering
Genomics
Neurodegenerative Diseases
Cytoplasm
Tissue engineering
Light
Neoplasms
Therapeutics

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Matsushita-Ishiodori, Y., Kuwabara, R., Sakakoshi, H., Endoh, T., & Ohtsuki, T. (2011). Photosensitizing carrier proteins for photoinducible RNA interference. Bioconjugate Chemistry, 22(11), 2222-2226. https://doi.org/10.1021/bc200095a

Photosensitizing carrier proteins for photoinducible RNA interference. / Matsushita-Ishiodori, Yuka; Kuwabara, Rina; Sakakoshi, Hiroyuki; Endoh, Tamaki; Ohtsuki, Takashi.

In: Bioconjugate Chemistry, Vol. 22, No. 11, 16.11.2011, p. 2222-2226.

Research output: Contribution to journalArticle

Matsushita-Ishiodori, Y, Kuwabara, R, Sakakoshi, H, Endoh, T & Ohtsuki, T 2011, 'Photosensitizing carrier proteins for photoinducible RNA interference', Bioconjugate Chemistry, vol. 22, no. 11, pp. 2222-2226. https://doi.org/10.1021/bc200095a
Matsushita-Ishiodori Y, Kuwabara R, Sakakoshi H, Endoh T, Ohtsuki T. Photosensitizing carrier proteins for photoinducible RNA interference. Bioconjugate Chemistry. 2011 Nov 16;22(11):2222-2226. https://doi.org/10.1021/bc200095a
Matsushita-Ishiodori, Yuka ; Kuwabara, Rina ; Sakakoshi, Hiroyuki ; Endoh, Tamaki ; Ohtsuki, Takashi. / Photosensitizing carrier proteins for photoinducible RNA interference. In: Bioconjugate Chemistry. 2011 ; Vol. 22, No. 11. pp. 2222-2226.
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