Photoreduction of pheophytin in photosystem II of the whole cells of green algae and cyanobacteria

V. V. Klimov, Suleyman Allakhverdiev, V. G. Ladygin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Photoreduction of Pheophytin 'a' (Pheo) accompanied by a decrease in the chlorophyll fluorescence yield is observed in photosystem II (PS II) of the whole cells of green algae Chlamydomonas reinhardii (a wild type and a mutant lacking both photosystem I and chlorophyll 'b'), Chlorella pyrenoidosa, Scenedesmus obliquus and cyanobacteria Phormidium laminosum, Anabaena variabilis and Cynechococcus elongatus under anaerobic conditions created by means of the glucose-glucoseoxidase-catalase. The photoreaction is activated by the addition of 1 μM CCCP, inhibited by 10 μM DCMU and reactivated upon subsequent addition of either ascorbate or dithionite. Oxidized NADP, benzyl viologen and methyl viologen accelerate dark oxidation of the reduced Pheo indicating that they are able to accept an electron from {Mathematical expression} in PS II. The data on both photoreduction of Pheo in the intact cells in the absence of exogenous reductants, when electron donation to reaction centers of PS II occurs only from water, and the inhibition of this photoreaction by DCMU, show that the Pheo photoreduction is sensitized by the reaction centers of PS II and probably occurs as a result of the electron donation from the water-splitting system being in the state S3, to {Mathematical expression} producing the long-lived state {Mathematical expression} and O2.

Original languageEnglish
Pages (from-to)355-363
Number of pages9
JournalPhotosynthesis Research
Volume10
Issue number3
DOIs
Publication statusPublished - Jan 1 1986
Externally publishedYes

Fingerprint

Pheophytins
Chlorophyta
Photosystem II Protein Complex
Cyanobacteria
Algae
photosystem II
Diuron
electrons
Electrons
Phormidium laminosum
Benzyl Viologen
Anabaena variabilis
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Scenedesmus
cells
Chlorella pyrenoidosa
Dithionite
Scenedesmus obliquus
chlorophyll
Photosystem I Protein Complex

Keywords

  • NADP reduction (Algae cells)
  • Pheophytin
  • Photosystem II
  • Reaction center
  • Water splitting

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Photoreduction of pheophytin in photosystem II of the whole cells of green algae and cyanobacteria. / Klimov, V. V.; Allakhverdiev, Suleyman; Ladygin, V. G.

In: Photosynthesis Research, Vol. 10, No. 3, 01.01.1986, p. 355-363.

Research output: Contribution to journalArticle

Klimov, V. V. ; Allakhverdiev, Suleyman ; Ladygin, V. G. / Photoreduction of pheophytin in photosystem II of the whole cells of green algae and cyanobacteria. In: Photosynthesis Research. 1986 ; Vol. 10, No. 3. pp. 355-363.
@article{94140bf9a2f64b518513e918460841fe,
title = "Photoreduction of pheophytin in photosystem II of the whole cells of green algae and cyanobacteria",
abstract = "Photoreduction of Pheophytin 'a' (Pheo) accompanied by a decrease in the chlorophyll fluorescence yield is observed in photosystem II (PS II) of the whole cells of green algae Chlamydomonas reinhardii (a wild type and a mutant lacking both photosystem I and chlorophyll 'b'), Chlorella pyrenoidosa, Scenedesmus obliquus and cyanobacteria Phormidium laminosum, Anabaena variabilis and Cynechococcus elongatus under anaerobic conditions created by means of the glucose-glucoseoxidase-catalase. The photoreaction is activated by the addition of 1 μM CCCP, inhibited by 10 μM DCMU and reactivated upon subsequent addition of either ascorbate or dithionite. Oxidized NADP, benzyl viologen and methyl viologen accelerate dark oxidation of the reduced Pheo indicating that they are able to accept an electron from {Mathematical expression} in PS II. The data on both photoreduction of Pheo in the intact cells in the absence of exogenous reductants, when electron donation to reaction centers of PS II occurs only from water, and the inhibition of this photoreaction by DCMU, show that the Pheo photoreduction is sensitized by the reaction centers of PS II and probably occurs as a result of the electron donation from the water-splitting system being in the state S3, to {Mathematical expression} producing the long-lived state {Mathematical expression} and O2.",
keywords = "NADP reduction (Algae cells), Pheophytin, Photosystem II, Reaction center, Water splitting",
author = "Klimov, {V. V.} and Suleyman Allakhverdiev and Ladygin, {V. G.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1007/BF00118301",
language = "English",
volume = "10",
pages = "355--363",
journal = "Photosynthesis Research",
issn = "0166-8595",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Photoreduction of pheophytin in photosystem II of the whole cells of green algae and cyanobacteria

AU - Klimov, V. V.

AU - Allakhverdiev, Suleyman

AU - Ladygin, V. G.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Photoreduction of Pheophytin 'a' (Pheo) accompanied by a decrease in the chlorophyll fluorescence yield is observed in photosystem II (PS II) of the whole cells of green algae Chlamydomonas reinhardii (a wild type and a mutant lacking both photosystem I and chlorophyll 'b'), Chlorella pyrenoidosa, Scenedesmus obliquus and cyanobacteria Phormidium laminosum, Anabaena variabilis and Cynechococcus elongatus under anaerobic conditions created by means of the glucose-glucoseoxidase-catalase. The photoreaction is activated by the addition of 1 μM CCCP, inhibited by 10 μM DCMU and reactivated upon subsequent addition of either ascorbate or dithionite. Oxidized NADP, benzyl viologen and methyl viologen accelerate dark oxidation of the reduced Pheo indicating that they are able to accept an electron from {Mathematical expression} in PS II. The data on both photoreduction of Pheo in the intact cells in the absence of exogenous reductants, when electron donation to reaction centers of PS II occurs only from water, and the inhibition of this photoreaction by DCMU, show that the Pheo photoreduction is sensitized by the reaction centers of PS II and probably occurs as a result of the electron donation from the water-splitting system being in the state S3, to {Mathematical expression} producing the long-lived state {Mathematical expression} and O2.

AB - Photoreduction of Pheophytin 'a' (Pheo) accompanied by a decrease in the chlorophyll fluorescence yield is observed in photosystem II (PS II) of the whole cells of green algae Chlamydomonas reinhardii (a wild type and a mutant lacking both photosystem I and chlorophyll 'b'), Chlorella pyrenoidosa, Scenedesmus obliquus and cyanobacteria Phormidium laminosum, Anabaena variabilis and Cynechococcus elongatus under anaerobic conditions created by means of the glucose-glucoseoxidase-catalase. The photoreaction is activated by the addition of 1 μM CCCP, inhibited by 10 μM DCMU and reactivated upon subsequent addition of either ascorbate or dithionite. Oxidized NADP, benzyl viologen and methyl viologen accelerate dark oxidation of the reduced Pheo indicating that they are able to accept an electron from {Mathematical expression} in PS II. The data on both photoreduction of Pheo in the intact cells in the absence of exogenous reductants, when electron donation to reaction centers of PS II occurs only from water, and the inhibition of this photoreaction by DCMU, show that the Pheo photoreduction is sensitized by the reaction centers of PS II and probably occurs as a result of the electron donation from the water-splitting system being in the state S3, to {Mathematical expression} producing the long-lived state {Mathematical expression} and O2.

KW - NADP reduction (Algae cells)

KW - Pheophytin

KW - Photosystem II

KW - Reaction center

KW - Water splitting

UR - http://www.scopus.com/inward/record.url?scp=0011599493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0011599493&partnerID=8YFLogxK

U2 - 10.1007/BF00118301

DO - 10.1007/BF00118301

M3 - Article

AN - SCOPUS:0011599493

VL - 10

SP - 355

EP - 363

JO - Photosynthesis Research

JF - Photosynthesis Research

SN - 0166-8595

IS - 3

ER -