Abstract
Octabromo-tetrakis(4-methylpyridyl)porphine (OBTMPyP), an octabromonated compound with 4 pyrole rings of tetrakis(4-methylpyridyl)porphine, selectively forms a complex with Cu2+ ions at pH 2.0. When 3.6×10(-5) mol/L OBTMPyP was added to the reaction mixture, the calibration curve showed good linearity for Cu2+ ions ranging from 0.01-2.2 μg (addition of 1.0 mL). A good coefficient of variation (Cu2+ ions=1.5 μg (addition of 1.0 mL), n=10, 0.8%) was obtained. The molar absorption coefficient (ε) based on Cu2+ ions was 8.5×10(4) L/mol•cm. This value was 6-fold greater than that determined with a clinical chemical analysis kit using the bathocuproine sulfonic acid method, which is a well-known method for spectrophotometric determination of the Cu2+ ion concentration. A deproteination method was successfully applied in the clinical analysis kit for determination of Cu2+ ion concentrations in control serum I, and the values determined using this method and the bathocuproine sulfonic acid method were almost the same.
| Original language | English |
|---|---|
| Pages (from-to) | 953-958 |
| Number of pages | 6 |
| Journal | Yakugaku Zasshi |
| Volume | 132 |
| Issue number | 8 |
| Publication status | Published - 2012 |
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Cite this
[Photometric determination of Cu2+ ion using octabromo-tetrakis(4-methylpyridyl)porphine]. / Mifune, Masaki; Iwado, Akimasa; Kamino, Shinichiro; Enomoto, Shuichi.
In: Yakugaku Zasshi, Vol. 132, No. 8, 2012, p. 953-958.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - [Photometric determination of Cu2+ ion using octabromo-tetrakis(4-methylpyridyl)porphine].
AU - Mifune, Masaki
AU - Iwado, Akimasa
AU - Kamino, Shinichiro
AU - Enomoto, Shuichi
PY - 2012
Y1 - 2012
N2 - Octabromo-tetrakis(4-methylpyridyl)porphine (OBTMPyP), an octabromonated compound with 4 pyrole rings of tetrakis(4-methylpyridyl)porphine, selectively forms a complex with Cu2+ ions at pH 2.0. When 3.6×10(-5) mol/L OBTMPyP was added to the reaction mixture, the calibration curve showed good linearity for Cu2+ ions ranging from 0.01-2.2 μg (addition of 1.0 mL). A good coefficient of variation (Cu2+ ions=1.5 μg (addition of 1.0 mL), n=10, 0.8%) was obtained. The molar absorption coefficient (ε) based on Cu2+ ions was 8.5×10(4) L/mol•cm. This value was 6-fold greater than that determined with a clinical chemical analysis kit using the bathocuproine sulfonic acid method, which is a well-known method for spectrophotometric determination of the Cu2+ ion concentration. A deproteination method was successfully applied in the clinical analysis kit for determination of Cu2+ ion concentrations in control serum I, and the values determined using this method and the bathocuproine sulfonic acid method were almost the same.
AB - Octabromo-tetrakis(4-methylpyridyl)porphine (OBTMPyP), an octabromonated compound with 4 pyrole rings of tetrakis(4-methylpyridyl)porphine, selectively forms a complex with Cu2+ ions at pH 2.0. When 3.6×10(-5) mol/L OBTMPyP was added to the reaction mixture, the calibration curve showed good linearity for Cu2+ ions ranging from 0.01-2.2 μg (addition of 1.0 mL). A good coefficient of variation (Cu2+ ions=1.5 μg (addition of 1.0 mL), n=10, 0.8%) was obtained. The molar absorption coefficient (ε) based on Cu2+ ions was 8.5×10(4) L/mol•cm. This value was 6-fold greater than that determined with a clinical chemical analysis kit using the bathocuproine sulfonic acid method, which is a well-known method for spectrophotometric determination of the Cu2+ ion concentration. A deproteination method was successfully applied in the clinical analysis kit for determination of Cu2+ ion concentrations in control serum I, and the values determined using this method and the bathocuproine sulfonic acid method were almost the same.
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UR - http://www.scopus.com/inward/citedby.url?scp=84865637969&partnerID=8YFLogxK
M3 - Article
C2 - 22864355
AN - SCOPUS:84865637969
VL - 132
SP - 953
EP - 958
JO - Yakugaku Zasshi
JF - Yakugaku Zasshi
SN - 0031-6903
IS - 8
ER -